Allele-specific, nested, one tube PCR:: application to Pfmdr1 polymorphisms in Plasmodium falciparum

被引：8

作者：

Adagu, IS ^{[1
]}

Warhurst, DC ^{[1
]}

机构：

[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Dis, London WC1E 7HT, England

来源：

PARASITOLOGY | 1999年 / 119卷

关键词：

allele-specific PCR; one tube semi-nested reaction; Pfmdr; 1; Plasmodium falciparum; malaria;

D O I：

10.1017/S0031182099004382

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

An allele-specific, one tube PCR for thr sensitive and reliable detection of point mutations in Plasmodium falciparum DNA is described. Design of specific internal primers and optimization Of the PCR is simple, and the procedure is robust and sensitive. Single nucleotide polymorphisms at codons 184, 1034, 1042 and 1246 of the P. falciparum multidrug resistance gene Pfmdr1, were examined in 6 laboratory isolates, to validate the technique.

引用

页码：1 / 6

页数：6

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