Cholesterol determination using protein-templated fluorescent gold nanocluster probes

被引：33

作者：

Chen, Xi ^{[1
]}

Baker, Gary A. ^{[1
]}

机构：

[1] Univ Missouri, Dept Chem, Columbia, MO 65211 USA

来源：

ANALYST | 2013年 / 138卷 / 24期

关键词：

STABILIZED AU NANOCLUSTERS; SENSOR; OXIDATION; IDENTIFICATION; BIOSENSOR; ELECTRODE; CLUSTERS;

D O I：

10.1039/c3an01548c

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We describe the development of a fluorescent biosensor platform for soluble cholesterol based on bovine serum albumin-stabilized gold nanocluster probes co-dissolved with cholesterol oxidase (ChOx) in a surfactant emulsion system. Selective enzymatic oxidation of cholesterol to cholest-4-en-3-one by ChOx produces stoichiometric amounts of H2O2 by-product, generating a quenching response signaling the presence of cholesterol at clinically relevant levels (LOD similar to 12 mu M).

引用

收藏

页码：7299 / 7302

页数：4

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