Quantitative one- and two-dimensional 13C spectra of microcrystalline proteins with enhanced intensity

被引：17

作者：

Purusottam, Rudra N. ^{[1
,2
,3
]}

Bodenhausen, Geoffrey ^{[1
,2
,3
]}

Tekely, Piotr ^{[1
,2
,3
]}

机构：

[1] Ecole Normale Super, Dept Chim, F-75005 Paris, France

[2] Univ Paris 06, F-75005 Paris, France

[3] CNRS, UMR 7203, Lab Biomol, F-75005 Paris, France

来源：

JOURNAL OF BIOMOLECULAR NMR | 2013年 / 57卷 / 01期

关键词：

Solid-state NMR; PARIS rf-irradiation; Microcrystalline proteins; Quantitative C-13 spectra; Spin diffusion; Heteronuclear Overhauser enhancement; SOLID-STATE NMR; OVERHAUSER EFFECT MEASUREMENTS; NUCLEAR-MAGNETIC-RESONANCE; SURFACTANT SYSTEMS; SPECTROSCOPY; POLARIZATION; RELAXATION; FIELDS; WATER;

D O I：

10.1007/s10858-013-9759-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We recorded quantitative, uniformly enhanced one- and two-dimensional C-13 spectra of labelled microcrystalline proteins. The approach takes advantage of efficient equilibration of magnetization by low-power proton irradiation using Phase Alternated Recoupling Irradiation Schemes and benefits simultaneously from uniform sensitivity enhancement due to efficient spin exchange that can overcome T-1(C-13) constraints and the presence of heteronuclear Overhauser effects.

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页码：11 / 19

页数：9

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