Improved quantification from 1H-NMR spectra using reduced repetition times

被引：34

作者：

Bharti, Santosh Kumar ^{[1
]}

Sinha, Neeraj ^{[1
]}

Joshi, Bhawani Shankar ^{[2
]}

Mandal, Sudhir Kumar ^{[1
]}

Roy, Raja ^{[1
]}

Khetrapal, Chunni Lal ^{[1
]}

机构：

[1] SGPGIMS Campus, Ctr Biomed Magnet Resonance, Lucknow 226014, Uttar Pradesh, India

[2] Bruker India Sci Pvt Ltd, Lucknow, Uttar Pradesh, India

来源：

METABOLOMICS | 2008年 / 4卷 / 04期

关键词：

T-1 and T-2 relaxation; CPMG; NMR spectroscopy; Body fluids; Metabolites; Quantitation;

D O I：

10.1007/s11306-008-0130-6

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

A method of choosing the correction factor based on Bloch equation for the quantitative estimation of metabolite from H-1 NMR spectra recorded with reduced recycle delay is prescribed. The procedure reduces the experimental time without substantially compromising the accuracy of quantitative estimation. It is based on choosing the correction factors, which depend on T-1 and T-2 of the metabolite and recycle delay used for recording the spectra. It is validated by studying a mixture of amino acids with known concentration of constituents and human serum sample and it provides accuracy of quantitative estimation to 95-96%.

引用

页码：367 / 376

页数：10

共 38 条

[1] Concentration measurement by proton NMR using the ERETIC method [J].

Akoka, S ;

Barantin, L ;

Trierweiler, M .

ANALYTICAL CHEMISTRY, 1999, 71 (13) :2554-2557

[2] 1H NMR spectroscopic method for diagnosis of malabsorption syndrome:: a pilot study [J].

Bala, L ;

Gowda, GAN ;

Ghoshal, UC ;

Misra, A ;

Bhandari, M ;

Khetrapal, CL .

NMR IN BIOMEDICINE, 2004, 17 (02) :69-75

[3] Malabsorption syndrome with and without small intestinal bacterial overgrowth:: A study on upper-gut aspirate using 1H NMR spectroscopy [J].

Bala, Lakshmi ;

Ghoshal, Uday Chand ;

Ghoshal, Ujjala ;

Tripathi, Pratima ;

Misra, Asha ;

Gowda, G. A. Nagana ;

Khetrapal, C. L. .

MAGNETIC RESONANCE IN MEDICINE, 2006, 56 (04) :738-744

[4]

Bell J D, 1989, NMR Biomed, V2, P246, DOI 10.1002/nbm.1940020513

[5] NUCLEAR-MAGNETIC-RESONANCE STUDIES OF BLOOD-PLASMA AND URINE FROM SUBJECTS WITH CHRONIC-RENAL-FAILURE - IDENTIFICATION OF TRIMETHYLAMINE-N-OXIDE [J].

BELL, JD ;

LEE, JA ;

LEE, HA ;

SADLER, PJ ;

WILKIE, DR ;

WOODHAM, RH .

BIOCHIMICA ET BIOPHYSICA ACTA, 1991, 1096 (02) :101-107

[6]

BELL JD, 1999, ENCYCLOPEDIA MAGNETI, V2, P989

[7]

BLOCH F, 1946, PHYS REV, V70, P460, DOI 10.1103/PhysRev.70.460

[8]

CARR HY, 1954, PHYS REV, V94, P638

[9] Precise and accurate quantitative 13C NMR with reduced experimental time [J].

Caytan, Elsa ;

Remaud, Gerald S. ;

Tenailleau, Eve ;

Akoka, Serge .

TALANTA, 2007, 71 (03) :1016-1021

[10] PROTON NMR-STUDIES OF BETAINE EXCRETION IN THE HUMAN NEONATE - CONSEQUENCES FOR CHOLINE AND METHYL-GROUP SUPPLY [J].

DAVIES, SEC ;

WOOLF, DA ;

CHALMERS, RA ;

RAFTER, JEM ;

ILES, RA .

JOURNAL OF NUTRITIONAL BIOCHEMISTRY, 1992, 3 (10) :523-530

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