Advantages of pentafluorophenylpropyl stationary phase over conventionial C18 stationary phase - Application to analysis of triamcinolone acetonide

被引：15

作者：

Havlikova, Lucie ^{[1
]}

Matysova, Ludmila ^{[1
]}

Hajkova, Renata ^{[1
]}

Satinsky, Dalibor ^{[1
]}

Solich, Petr ^{[1
]}

机构：

[1] Charles Univ Prague, Fac Pharm, Dept Analyt Chem, Hradec Kralove 50005, Czech Republic

来源：

TALANTA | 2008年 / 76卷 / 03期

关键词：

degradation product; C18; columns; HPLC; pentafluorophenylpropyl stationary phase; triamcinolone acetonide;

D O I：

10.1016/j.talanta.2008.03.048

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A pentafluorophenylpropyl (PFPP) stationary phase was for the first time tested for the simultaneous determination of triamcinolone acetonide, its degradation product triamcinolone and two preservatives, methylparaben, and propylparaben. A new simple isocratic reversed phase HPLC method with UV detection, using estradiol hemihydrate as an internal standard, has been developed and validated. Chromatography was performed on a Discovery HS F5 column (150 mm x 4.6 mm, 5 mu m) using a binary mobile phase composed of acetonitrile and water 45:55 (v:v). The flow-rate was 0.6 mL/min, the column temperature 25 degrees C and the UV detection was accomplished at 240 nm. The chromatography results using PFPP stationary phase were compared with those obtained using conventional C18 columns. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：597 / 601

页数：5

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