A bacterial cloning vector using a mutated Aequorea green fluorescent protein as an indicator

被引：39

作者：

Inouye, S

Ogawa, H

Yasuda, K

Umesono, K

Tsuji, FI

机构：

[1] UNIV CALIF SAN DIEGO, SCRIPPS INST OCEANOG, MARINE BIOL RES DIV 0202, SAN DIEGO, CA 92093 USA

[2] NARA INST SCI & TECHNOL, GRAD SCH BIOL SCI, IKOMA, NARA 63001, JAPAN

来源：

GENE | 1997年 / 189卷 / 02期

关键词：

recombinant DNA; gene expression; temperature dependence; reporter gene; pBluescript;

D O I：

10.1016/S0378-1119(96)00753-6

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The bacterial cloning vector, pGreenscript A, derived from the mutated Aequorea green fluorescent protein (GFP-S65A) gene, when expressed in E. coli produced colonies that showed yellow color under daylight and strong green fluorescence under long-wave ultraviolet light. The vector was used to select for inserted foreign genes based on the loss of the yellow color/green fluorescence of E. coli cells caused by the insertional inactivation of GFP production. (C) 1997 Elsevier Science B.V.

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页码：159 / 162

页数：4

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