Alteration by lead acetate of rat submandibular gland morphology and ultrastructure

被引:7
作者
Abdollahi, M
Sharifzadeh, M
Marzban, H
Abri, G
Torab-Jahromi, M
机构
[1] Univ Tehran Med Sci, Fac Pharm, Dept Pharmacol & Toxicol, Tehran 141556451, Iran
[2] Univ Tehran Med Sci, Dept Anat & Pathol, Tehran, Iran
关键词
lead acetate; submandibular; ultrastructure; rat;
D O I
10.1080/107691899229106
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Structural changes of rat submandibular glands (SMG) after long-term treatment with various doses of lead (0.01%, 0.04%, 0.05%) were investigated in this study. After termination of treatment, SMGs were removed and homogenized for measurement of total protein, DNA, calcium, and lead concentrations. Also ultrastructural examination of glands by electron, microscopy was carried out. Total protein, DNA, and intracellular calcium concentrations of treated glands at 0.04% and 0.05% lead concentrations showed significant reduction when compared with those of controls. Data of lead measurement, as determined by atomic absorption spectroscopy, showed that lead treatment resulted in accumulation of lead in rats' SMGs. Lead treatment also caused degenerative changes in the cells. Dilation of rough endoplasmic reticulum (RER) and swollen, mitochondria were observed in the (0.05%) treated group. Transformation and enlargement of mitochondria also were determined in the (0.04%) treated group. We propose that Lead treatment can reduce exocytosis processes by the reduction, in energy level of the cell and destruction of RER or mitochondria. Considering properties of lead to substitute for calcium in many intracellular events different types of alterations can result, including interaction with protein and DNA synthesis. To elucidate the exact mechanisms of observed alterations requires further studies.
引用
收藏
页码:139 / 147
页数:9
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