Disulfide-Linked, Gold Nanoparticle Based Reagent for Detecting Small Molecular Weight Thiols

被引：83

作者：

Durocher, Suzanne ^{[1
]}

Rezaee, Asad ^{[3
]}

Hamm, Caroline ^{[1
]}

Rangan, Chitra ^{[2
]}

Mittler, Silvia ^{[3
]}

Mutus, Bulent ^{[1
]}

机构：

[1] Univ Windsor, Dept Chem & Biochem, Windsor, ON N9B 3P4, Canada

[2] Univ Windsor, Dept Phys, Windsor, ON N9B 3P4, Canada

[3] Univ Western Ontario, Dept Phys & Astron, London, ON, Canada

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 07期

基金：

加拿大自然科学与工程研究理事会;

关键词：

CAPILLARY-ELECTROPHORESIS; FLUORESCENCE DETECTION; HUMAN PLASMA; LIQUID-CHROMATOGRAPHY; HYDROGEN-SULFIDE; REDOX REGULATION; CYSTEINE; HOMOCYSTEINE; ROLES; GLUTATHIONE;

D O I：

10.1021/ja808548x

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Disulfide-linked gold nanoparticles (AuNP) were synthesized by reacting dithiobis[succinimidylpropionate] (DSP) coated nanoparticles with glutathione disulfide. AuNP-cross-linking was monitored by the red shift and broadening of the AuNP's localized surface plasmon absorption resonance (LSPR) spectrum. The exposure of the disulfide-linked AuNPs to a variety of free thiols with systematically varying molecular weight revealed a AuNP-disulfide stability to reduction by thiols up to a critical molecular weight, M-c, of >300 Da thus making the disulfide-linked AuNP the first reagent that can discriminate thiols based on their size.

引用

页码：2475 / +

页数：4

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