PDMP blocks the BFA-induced ADP-ribosylation of BARS-50 in isolated Golgi membranes

被引：7

作者：

De Matteis, MA

Luna, A

Di Tullio, G

Corda, D

Kok, JW

Luini, A

Egea, G

机构：

[1] Univ Barcelona, IDIBAPS, Fac Med, Dept Cellular Biol Anat Patol, E-08036 Barcelona, Spain

[2] Ist Ric Farmacol Mario Negri, Consorzio Mario Negri Sud, Dept Cell Biol & Oncol, I-66030 Santa Maria Imbaro, Chieti, Italy

[3] Univ Groningen, Dept Physiol Chem, NL-9713 AV Groningen, Netherlands

来源：

FEBS LETTERS | 1999年 / 459卷 / 03期

关键词：

Golgi complex; ADP-ribosylation; D; L-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol; brefeldin A; membrane transport;

D O I：

10.1016/S0014-5793(99)01269-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We reported that an inhibitor of sphingolipid biosynthesis, D,L-threo-1-phenyl-2-decanoylamino-3-morpholinol-1-propanol (PDMP), blocks brefeldin A (BFA)-induced retrograde membrane transport from the Golgi complex to the endoplasmic reticulum (ER) (Kok et al,, 1998, J. Cell Biol. 142, 25-38), We now show that PDMP partially blocks the BFA-induced ADP-ribosylation of the cytosolic protein BARS-50. Moreover, PDMP does not interfere with the BFA-induced inhibition of the binding of ADP-ribosylation factor (ARF) and the coatomer component p-coat protein to Golgi membranes, These results are consistent with a role of ADP-ribosylation in the action of BFA and with the involvement of BARS-50 in the regulation of membrane trafficking. (C) 1999 Federation of European Biochemical Societies.

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页码：310 / 312

页数：3

相关论文

共 23 条

[1] STIMULATION OF ENDOGENOUS ADP-RIBOSYLATION BY BREFELDIN-A [J].

DEMATTEIS, MA ;

DIGIROLAMO, M ;

COLANZI, A ;

PALLAS, M ;

DITULLIO, G ;

MCDONALD, LJ ;

MOSS, J ;

SANTINI, G ;

BANNYKH, S ;

CORDA, D ;

LUINI, A .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1994, 91 (03) :1114-1118

[2] RECEPTOR AND PROTEIN KINASE-C-MEDIATED REGULATION OF ARF BINDING TO THE GOLGI-COMPLEX [J].

DEMATTEIS, MA ;

SANTINI, G ;

KAHN, RA ;

DITULLIO, G ;

LUINI, A .

NATURE, 1993, 364 (6440) :818-821

[3] EVIDENCE THAT THE 50-KDA SUBSTRATE OF BREFELDIN A-DEPENDENT ADP-RIBOSYLATION BINDS GTP AND IS MODULATED BY THE G-PROTEIN BETA-GAMMA SUBUNIT COMPLEX [J].

DIGIROLAMO, M ;

SILLETTA, MG ;

DEMATTEIS, MA ;

BRACA, A ;

COLANZI, A ;

PAWLAK, D ;

RASENICK, MM ;

LUINI, A ;

CORDA, D .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (15) :7065-7069

[4] BREFELDIN-A REDISTRIBUTES RESIDENT AND ITINERANT GOLGI PROTEINS TO THE ENDOPLASMIC-RETICULUM [J].

DOMS, RW ;

RUSS, G ;

YEWDELL, JW .

JOURNAL OF CELL BIOLOGY, 1989, 109 (01) :61-72

[5] BREFELDIN-A INHIBITS GOLGI MEMBRANE-CATALYZED EXCHANGE OF GUANINE-NUCLEOTIDE ONTO ARF PROTEIN [J].

DONALDSON, JG ;

FINAZZI, D ;

KLAUSNER, RD .

NATURE, 1992, 360 (6402) :350-352

[6] BINDING OF ARF AND BETA-COP TO GOLGI MEMBRANES - POSSIBLE REGULATION BY A TRIMERIC G-PROTEIN [J].

DONALDSON, JG ;

KAHN, RA ;

LIPPINCOTTSCHWARTZ, J ;

KLAUSNER, RD .

SCIENCE, 1991, 254 (5035) :1197-1199

[7] The curious status of the Golgi apparatus [J].

Glick, BS ;

Malhotra, V .

CELL, 1998, 95 (07) :883-889

[8] INHIBITION BY BREFELDIN-A OF A GOLGI MEMBRANE ENZYME THAT CATALYZES EXCHANGE OF GUANINE-NUCLEOTIDE BOUND TO ARF [J].

HELMS, JB ;

ROTHMAN, JE .

NATURE, 1992, 360 (6402) :352-354

[9] THE BREFELDIN A-INDUCED RETROGRADE TRANSPORT FROM THE GOLGI-APPARATUS TO THE ENDOPLASMIC-RETICULUM DEPENDS ON CALCIUM SEQUESTERED TO INTRACELLULAR STORES [J].

IVESSA, NE ;

DELEMOSCHIARANDINI, C ;

GRAVOTTA, D ;

SABATINI, DD ;

KREIBICH, G .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1995, 270 (43) :25960-25967

[10] BREFELDIN-A - INSIGHTS INTO THE CONTROL OF MEMBRANE TRAFFIC AND ORGANELLE STRUCTURE [J].

KLAUSNER, RD ;

DONALDSON, JG ;

LIPPINCOTTSCHWARTZ, J .

JOURNAL OF CELL BIOLOGY, 1992, 116 (05) :1071-1080