An 8-hour system for Salmonella detection with immunomagnetic separation and homogeneous time-resolved fluorescence PCR

被引：30

作者：

Hagren, Virve ^{[1
]}

von Lode, Piia ^{[1
]}

Syrjala, Anniina ^{[1
]}

Korpimaki, Teemu ^{[2
]}

Tuomola, Mika ^{[2
]}

Kauko, Otto ^{[1
]}

Nurmi, Jussi ^{[1
]}

机构：

[1] Abascus Diagnost Ltd, FI-20520 Turku, Finland

[2] Raisio Diagnost, FI-20520 Turku, Finland

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2008年 / 125卷 / 02期

关键词：

Salmonella; immunomagnetic separation; homogeneous assay; PCR; time-resolved fluorometry; dry chemistry;

D O I：

10.1016/j.ijfoodmicro.2008.03.037

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

We describe a system consisting of rapid sample enrichment and homogeneous end-point PCR analysis that enables the detection of Salmonella in various food matrices in 8 h. Sample preparation starts with 6 h enrichment step in supplemented broth, after which Salmonella cells are collected with immunomagnetic particles. The particles are washed and dispensed to ready-to-use PCR reaction vessels, which contain dried assay-specific reagents and an internal amplification control. PCR is performed with a novel instrument platform utilising the sensitive label technology of time-resolved fluorometry. Qualitative assay results are automatically interpreted and available in 45 min after sample addition. The overall accuracy, sensitivity and specificity of the Magda(TM) CA Salmonella system were 99.1%, 98.4% and 100.0%, respectively, based on the evaluation of 107 samples (beef, pork, poultry and ready-to-eat meals) artificially contaminated with sublethally injured Salmonella cells. (C) 2008 Elsevier B.V. All rights reserved.

引用

页码：158 / 161

页数：4

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