Molecular cloning of cDNAs encoding hypoxia-inducible factor (HIF)-1α and-2α of bovine arterial endothelial cells

被引：16

作者：

Hara, S ^{[1
]}

Kobayashi, C ^{[1
]}

Imura, N ^{[1
]}

机构：

[1] Kitasato Univ, Sch Pharmaceut Sci, Dept Publ Hlth, Minato Ku, Tokyo 1088641, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1999年 / 1445卷 / 02期

关键词：

hypoxia; endothelial cell; transcription factor; hypoxia inducible factor; basic helix-loop-helix/PAS domain; bovine;

D O I：

10.1016/S0167-4781(99)00048-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Hypoxia-inducible factor (HIF)-l alpha and -2 alpha are two basic helix-loop-helix/PAS domain transcriptional factors that mediate hypoxia-induced gene expression. We found that bovine arterial endothelial cells (BAEC) expressed both HIF-1 alpha and -2 alpha by RT-PCR and then isolated cDNAs encoding these two transcriptional factors of BAEC. The deduced amino acid sequences of both HIF-1 alpha and -2 alpha showed high homologies among mammalian species. Northern blot analysis indicated that the mRNAs for HIF-1 alpha and -2 alpha from BAEC showed a size of approx. 5.5 and 6.2 kilobases, respectively and that both mRNAs were constitutively expressed and not induced by hypoxia in BAEC. (C) 1999 Elsevier Science B.V. All rights reserved.

引用

页码：237 / 243

页数：7

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