Chemical hydrolysis of DOTAP and DOPE in a liposomal environment

被引：14

作者：

Vernooij, EAAM

Kettenes-van den Bosch, JJ

Underberg, WJM

Crommelin, DJA

机构：

[1] Univ Utrecht, UIPS, Dept Biomol Mass Spectrometry, NL-3508 TB Utrecht, Netherlands

[2] Univ Utrecht, Bijvoet Ctr Biomol Res, NL-3508 TB Utrecht, Netherlands

[3] Univ Utrecht, UIPS, Fac Pharm, Dept Pharmaceut, NL-3508 TB Utrecht, Netherlands

[4] Univ Utrecht, UIPS, Fac Pharm, Dept Pharmaceut Anal, NL-3508 TB Utrecht, Netherlands

[5] OctoPlus BV, NL-2333 CL Leiden, Netherlands

来源：

JOURNAL OF CONTROLLED RELEASE | 2002年 / 79卷 / 1-3期

关键词：

phospholipids; hydrolysis; cationic liposomes; DOTAP; DOPE;

D O I：

10.1016/S0168-3659(01)00534-X

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

In this study the hydrolysis kinetics of I,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC), 1,2-dioleoyl-sn-glycerow-3-phosphoethanolamine (DOPE) and 1,2-dioleoyltrimethylammoniumpropane (DOTAP) in net neutral DPPC-DOPE (3:1, mol/mol) and cationic DOTAP-DOPE (1:1, mol/mol) liposomes are described. The log k(obs)-pH profile for DOTAP-DOPE liposomes differs markedly From earlier observed hydrolysis profiles: the slope approaches zero in the acidic region and + 1 in the alkaline region. The concept of amine-influenced hydrolysis is introduced to explain the lack of pH dependency in the acidic region of the log k(obs)-pH profiles. (C) 2002 Elsevier Science B.V. All rights reserved.

引用

页码：299 / 303

页数：5

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[4]

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