Rat bone marrow stromal cells-seeded porous gelatin/tricalcium phosphate/oligomeric proanthocyanidins composite scaffold for bone repair

被引:20
作者
Chen, Kuo-Yu [1 ]
Chung, Chia-Mei [2 ]
Chen, Yueh-Sheng [3 ,4 ]
Bau, Da-Tian [5 ]
Yao, Chun-Hsu [3 ,4 ]
机构
[1] Natl Yunlin Univ Sci & Technol, Dept Chem & Mat Engn, Yunlin, Taiwan
[2] Cent Taiwan Univ Sci & Technol, Inst Biomed Engn & Mat Sci, Taichung, Taiwan
[3] China Med Univ, Dept Biomed Imaging & Radiol Sci, Taichung 40402, Taiwan
[4] China Med Univ, Sch Chinese Med, Taichung 40402, Taiwan
[5] China Med Univ & Hosp, Terry Fox Canc Res Lab, Taichung, Taiwan
关键词
bone tissue engineering; porous; bone marrow stromal cells; oligomeric proanthocyanidins; gelatin; tricalcium phosphate; BETA-TRICALCIUM PHOSPHATE; MESENCHYMAL STEM-CELLS; CROSS-LINKED GELATIN; OSTEOGENIC DIFFERENTIATION; CRANIAL RECONSTRUCTION; IN-VITRO; HYDROXYAPATITE; LINKING; DEFECT; PROLIFERATION;
D O I
10.1002/term.1461
中图分类号
Q813 [细胞工程];
学科分类号
100113 [医学细胞生物学];
摘要
Repair of bone defects remains a major challenge in orthopaedic surgery. Bone tissue engineering is an attractive approach for treating bone loss in various shapes and amounts. The aim of this study was to prepare and evaluate the feasibility of a porous scaffold, which was composed of oligomeric proanthocyanidin crosslinked gelatin mixed with -tricalcium phosphate (GTP) and was seeded with bone marrow stromal cells (BMSCs) as a bone substitute. GTP scaffolds were made porous using a salt-leaching method. The physicochemical properties of the scaffold were evaluated to determine the optimal salt:composite weight ratio. The results indicated that the GTP scaffold had a favourable macroporous structure and higher porosity when the salt:composite weight ratio was 4:1. Cytotoxic tests demonstrated that extracts from the GTP scaffolds promoted the proliferation of BMSCs. Rat BMSCs were seeded on a GTP scaffold and cultured in a spinner flask. After 2weeks of culture, scanning electron microscopy observation showed that the cells adhered well to the surfaces of the pores in the scaffold. Moreover, this study explored the biological response of rat calvarial bone to the scaffold to evaluate its potential in bone tissue engineering. Bone defects were filled with BMSC-seeded GTP scaffold and acellular GTP scaffold. After 8weeks, the scaffold induced new bone formation at a bone defect, as was confirmed by X-ray microradiography and histology. The BMSC-seeded scaffold induced more new bone formation than did an acellular scaffold. These observations suggest that the BMSCs-seeded GTP scaffold can promote the regeneration of defective bone tissue. Copyright (c) 2012 John Wiley & Sons, Ltd.
引用
收藏
页码:708 / 719
页数:12
相关论文
共 41 条
[1]
Preparation of Porous Biphasic Calcium Phosphate-Gelatin Nanocomposite for Bone Tissue Engineering [J].
Bakhtiari, L. ;
Rezai, H. R. ;
Hosseinalipour, S. M. ;
Shokrgozar, M. A. .
JOURNAL OF NANO RESEARCH, 2010, 11 :67-72
[2]
Proliferation and osteogenic differentiation of human bone marrow stromal cells on alginate-getatine-hydroxyapatite scaffolds with anisotropic pore structure [J].
Bernhardt, A. ;
Despang, F. ;
Lode, A. ;
Demmler, A. ;
Hanke, T. ;
Gelinsky, M. .
Journal of Tissue Engineering and Regenerative Medicine, 2009, 3 (01) :54-62
[3]
Effect of added gelatin on the properties of calcium phosphate cement [J].
Bigi, A ;
Bracci, B ;
Panzavolta, S .
BIOMATERIALS, 2004, 25 (14) :2893-2899
[4]
Bruder SP, 1998, CLIN ORTHOP RELAT R, pS247
[5]
The effect of implants loaded with autologous mesenchymal stem cells on the healing of canine segmental bone defects [J].
Bruder, SP ;
Kraus, KH ;
Goldberg, VM ;
Kadiyala, S .
JOURNAL OF BONE AND JOINT SURGERY-AMERICAN VOLUME, 1998, 80A (07) :985-996
[6]
Effect of Use of Slow Release of Bone Morphogenetic Protein-2 and Transforming Growth Factor-Beta-2 in a Chitosan Gel Matrix on Cranial Bone Graft Survival in Experimental Cranial Critical Size Defect Model [J].
Canter, Halil Ibrahim ;
Vargel, Ibrahim ;
Korkusuz, Petek ;
Oner, Filiz ;
Gungorduk, Dilsad B. ;
Cil, Barbaros ;
Karabulut, Erdem ;
Sargon, Mustafa F. ;
Erk, Yucel .
ANNALS OF PLASTIC SURGERY, 2010, 64 (03) :342-350
[7]
A biodegradable porous composite scaffold of PGA/β-TCP for bone tissue engineering [J].
Cao, Hong ;
Kuboyama, Noboru .
BONE, 2010, 46 (02) :386-395
[8]
Novel Bone Substitute Composed of Oligomeric Proanthocyanidins-Crosslinked Gelatin and Tricalcium Phosphate [J].
Chen, Kuo-Yu ;
Shyu, Pei-Chi ;
Chen, Yueh-Sheng ;
Yao, Chun-Hsu .
MACROMOLECULAR BIOSCIENCE, 2008, 8 (10) :942-950
[9]
Reconstruction of calvarial defect using a tricalcium phosphate-oligomeric proanthocyanidins cross-linked gelatin composite [J].
Chen, Kuo-Yu ;
Shyu, Pei-Chi ;
Dong, Guo-Chung ;
Chen, Yueh-Sheng ;
Kuo, Wei-Wen ;
Yao, Chun-Hsu .
BIOMATERIALS, 2009, 30 (09) :1682-1688
[10]
BIOCERAMICS CONSISTING OF CALCIUM-PHOSPHATE SALTS [J].
DEGROOT, K .
BIOMATERIALS, 1980, 1 (01) :47-50