Mannosylphosphodolichol synthase activity is associated with a 32 kDa phosphoprotein

被引：10

作者：

Banerjee, DK ^{[1
]}

DaSilva, JJ ^{[1
]}

Bigio, B ^{[1
]}

机构：

[1] Univ Puerto Rico, Sch Med, Dept Biochem, San Juan, PR 00936 USA

来源：

BIOSCIENCE REPORTS | 1999年 / 19卷 / 03期

关键词：

Dol-P-Man synthase; N-linked glycoprotein; phosphoprotein; oligosaccharide-PP-Dol; beta-adrenoreceptor; 3; 5 '-cyclic adenosine monophosphate; endothelial cells;

D O I：

10.1023/A:1020221602373

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Failure of actinomycin D to block the activation of Dol-P-Man synthase in isoproterenol-treated capillary endothelial cells, supported that isoproterenol effect was not mediated by active transcription of the Dol-P-Man synthase gene during a short-term beta-adrenoreceptor stimulation. Instead, it was a net effect of protein phosphorylation by cAMP-dependent protein kinase. Using antibody as a probe we have now demonstrated that Dol-P-Man synthase activity is associated with a 32 kDa ER phosphoprotein.

引用

页码：169 / 177

页数：9

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