Biological function evaluation and effects of laser micro-pore burn-denatured acellular dermal matrix

被引:13
作者
Zhang, Youlai [1 ,2 ]
Zeng, Yuanlin [2 ]
Xin, Guohua [2 ]
Zou, Lijin [2 ]
Ding, Yuewei [2 ]
Jiang, Duyin [3 ,4 ]
机构
[1] Shandong Univ, Grad Sch Jinan, Jinan 250100, Shandong, Peoples R China
[2] Nanchang Univ, Affiliate Hosp 1, Burn Ctr, Nanchang 330006, Jiangxi, Peoples R China
[3] Shandong Univ, Hosp 2, Dept Emergency, Jinan 250033, Shandong, Peoples R China
[4] Shandong Univ, Hosp 2, Dept Burns & Plast Surg, Jinan 250033, Shandong, Peoples R China
关键词
Burn; Wound healing; Laser micro-pore technique; Denatured dermis; Acellular dermal matrix; VASCULAR-PERMEABILITY; MODEL; SKIN;
D O I
10.1016/j.burns.2017.07.009
中图分类号
R4 [临床医学];
学科分类号
100218 [急诊医学];
摘要
Objective: In the field of burns repairs, many problems exist in the shortage of donor skin, the expense of allograft or xenograft skin, temporary substitution and unsatisfactory extremity function after wound healing. Previous studies showed that burn-denatured skin could return to normal dermis formation and function. This study investigates the application of laser micro-pore burn-denatured acellular dermis matrix (DADM) from an escharotomy in the repair of burn wounds and evaluates the biological properties and wound repair effects of DADM in implantation experiments in Kunming mice. Methods: Specific-pathogen-free (SPF) Kunming mice were used in this study. A deep II degrees burn wound was created on the dorsum of the mice by an electric heated water bath. The full-thickness wound tissue was harvested. The necrotic tissue and subcutaneous tissue were removed. The denatured dermis was preserved and treated with 0.25% trypsin, 0.5% Triton X-100. The DADM was drilled by laser micro-pore. The biological properties and grafting effects of laser micro-pore burn-DADM were evaluated by morphology, cytokine expression levels and subcutaneous implantation experiments in Kunming mice. Results: We found statistical significance (P<0.05) of the elastic modulus (MPa), maximum load force (N) and contraction measurement (CM) of the laser micro-pore burn-DADM (experimental group) compared to the control group (no laser micro-pore burn-DADM). Cytokine expression level was different in the dermal matrixes harvested at various time points after burn (24h, 48h, 72h and infected wound group). Comparing the dermal matrix from 24h burn tissue to infected wound tissue, the expression level of IL-6, MMP-24, VE-cadherin and VEGF were decreased. We found no inflammatory cells infiltration in the dermal matrix were observed in both experimental and control groups (24h burn group), while the obviously vascular infiltration and fiber fusion were observed in the experimental group after subcutaneous implantation experiments. Conclusion: There was better bio-performance, low immunogenicity and better dermal incorporation after treated by laser micro-pore drilling and decellularized deep II degrees burn-DADM, which may be considered as a better substitute for dermal matrix. Furthermore, the earlier harvested DADM after burn (24h) shows the better transplantation effect. (c) 2017 Elsevier Ltd and ISBI. All rights reserved.
引用
收藏
页码:350 / 358
页数:9
相关论文
共 20 条
[1]
MT5-MMP is a new pro-amyloidogenic proteinase that promotes amyloid pathology and cognitive decline in a transgenic mouse model of Alzheimer's disease [J].
Baranger, Kevin ;
Marchalant, Yannick ;
Bonnet, Amandine E. ;
Crouzin, Nadine ;
Carrete, Alex ;
Paumier, Jean-Michel ;
Py, Nathalie A. ;
Bernard, Anne ;
Bauer, Charlotte ;
Charrat, Eliane ;
Moschke, Katrin ;
Seiki, Mothoharu ;
Vignes, Michel ;
Lichtenthaler, Stefan F. ;
Checler, Frederic ;
Khrestchatisky, Michel ;
Rivera, Santiago .
CELLULAR AND MOLECULAR LIFE SCIENCES, 2016, 73 (01) :217-236
[2]
Genetically modified porcine split-thickness skin grafts as an alternative to allograft for provision of temporary wound coverage: preliminary characterization [J].
Barone, Angelo A. Leto ;
Mastroianni, Melissa ;
Farkash, Evan A. ;
Mallard, Christopher ;
Albritton, Alexander ;
Torabi, Radbeh ;
Leonard, David A. ;
Kurtz, Josef M. ;
Sachs, David H. ;
Cetrulo, Curtis L., Jr. .
BURNS, 2015, 41 (03) :565-574
[3]
Preparation of laser micropore porcine acellular dermal matrix for skin graft: An experimental study [J].
Chai Jia-Ke ;
Liang Li-Ming ;
Yang Hong-Ming ;
Feng Rui ;
Yin Hui-Nan ;
Li Feng-Yu ;
Sheng Zhi-Yong .
BURNS, 2007, 33 (06) :719-725
[4]
VE-cadherin lets it flow [J].
Du Toit, Andrea .
NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2015, 16 (05) :268-268
[5]
Association between IL-6-174G/C Polymorphism and the Risk of Sepsis and Mortality: A Systematic Review and Meta-Analysis [J].
Gao, Jun-wei ;
Zhang, An-qiang ;
Pan, Wei ;
Yue, Cai-li ;
Zeng, Ling ;
Gu, Wei ;
Jiang, Jianxin .
PLOS ONE, 2015, 10 (03)
[6]
Crosslinking and composition influence the surface properties, mechanical stiffness and cell reactivity of collagen-based films [J].
Grover, Chloe N. ;
Gwynne, Jessica H. ;
Pugh, Nicholas ;
Hamaia, Samir ;
Farndale, Richard W. ;
Best, Serena M. ;
Cameron, Ruth E. .
ACTA BIOMATERIALIA, 2012, 8 (08) :3080-3090
[7]
VE-cadherin: at the front, center, and sides of endothelial cell organization and function [J].
Harris, Elizabeth S. ;
Nelson, W. James .
CURRENT OPINION IN CELL BIOLOGY, 2010, 22 (05) :651-658
[8]
Thrombomodulin blocks calcineurin inhibitor-induced vascular permeability via inhibition of Src/VE-cadherin axis [J].
Ikezoe, T. ;
Yang, J. ;
Nishioka, C. ;
Umezawa, K. ;
Yokoyama, A. .
BONE MARROW TRANSPLANTATION, 2017, 52 (02) :245-251
[9]
Lycorine hydrochloride inhibits metastatic melanoma cell-dominant vasculogenic mimicry [J].
Liu, Ruifang ;
Cao, Zhifei ;
Tu, Jian ;
Pan, Yanyan ;
Shang, Bingxue ;
Zhang, Gaochuan ;
Bao, Meimei ;
Zhang, Shasha ;
Yang, Ping ;
Zhou, Quansheng .
PIGMENT CELL & MELANOMA RESEARCH, 2012, 25 (05) :630-638
[10]
Luo X, 2012, CHIN J INJ REPAIR WO, V7, P240