Real-time centrosome reorientation during fibroblast migration

被引：16

作者：

Gomes, ER ^{[1
]}

Gundersen, GG ^{[1
]}

机构：

[1] Columbia Univ, Dept Anat & Cell Biol, New York, NY 10027 USA

来源：

METHODS IN ENZYMOLOGY, VOL 406, REGULATORS AND EFFECTORS OF SMALL GTPASES: RHO FAMILY | 2006年 / 406卷

关键词：

D O I：

10.1016/S0076-6879(06)06045-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The centrosome is positioned between the nucleus and the leading edge of many types of migrating cells. Cdc42 regulates this centrosome reorientation through its effectors Par6 and MRCK. Using time-lapse microscopy of live cells, the mechanisms and kinetics of centrosome reorientation can be studied. In this chapter, we describe a modification in the standard wound healing assay that allows the study of signaling pathways involved in centrosome reorientation and other polarization events that occur before cell migration. We also describe a method for visualization of centrosome reorientation by time-lapse microscopy using NIH 3T3 fibroblasts stably transfected with GFP-tubulin.

引用

页码：579 / 592

页数：14

共 25 条

[1] MEMBRANE INSERTION AT THE LEADING-EDGE OF MOTILE FIBROBLASTS [J].

BERGMANN, JE ;

KUPFER, A ;

SINGER, SJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1983, 80 (05) :1367-1371

[2] Rho guanosine triphosphatase mediates the selective stabilization of microtubules induced by lysophosphatidic acid [J].

Cook, TA ;

Nagasaki, T ;

Gundersen, GG .

JOURNAL OF CELL BIOLOGY, 1998, 141 (01) :175-185

[3] Cdc42 - the centre of polarity [J].

Etienne-Manneville, S .

JOURNAL OF CELL SCIENCE, 2004, 117 (08) :1291-1300

[4] Integrin-mediated activation of Cdc42 controls cell polarity in migrating astrocytes through PKCζ [J].

Etienne-Manneville, S ;

Hall, A .

CELL, 2001, 106 (04) :489-498

[5] MECHANISM OF CENTROSOME POSITIONING DURING THE WOUND RESPONSE IN BSC-1 CELLS [J].

EUTENEUER, U ;

SCHLIWA, M .

JOURNAL OF CELL BIOLOGY, 1992, 116 (05) :1157-1166

[6] Nuclear movement regulated by Cdc42, MRCK, myosin, and actin flow establishes MTOC polarization in migrating cells [J].

Gomes, ER ;

Jani, S ;

Gundersen, GG .

CELL, 2005, 121 (03) :451-463

[7] DISTRIBUTION OF MICROTUBULE ORGANIZING CENTERS IN MIGRATING SHEETS OF ENDOTHELIAL-CELLS [J].

GOTLIEB, AI ;

MAY, LM ;

SUBRAHMANYAN, L ;

KALNINS, VI .

JOURNAL OF CELL BIOLOGY, 1981, 91 (02) :589-594

[8]

GREGORY WA, 1988, J NEUROSCI, V8, P1728

[9]

GUNDERSEN GG, 1994, J CELL SCI, V107, P645

[10] SELECTIVE STABILIZATION OF MICROTUBULES ORIENTED TOWARD THE DIRECTION OF CELL-MIGRATION [J].

GUNDERSEN, GG ;

BULINSKI, JC .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (16) :5946-5950

← 1 2 3 →