Modern applications of analytical ultracentrifugation

被引：219

作者：

Laue, TM ^{[1
]}

Stafford, WF

机构：

[1] Univ New Hampshire, Dept Biochem & Mol Biol, Durham, NH 03824 USA

[2] Boston Biomed Res Inst, Boston, MA 02114 USA

来源：

ANNUAL REVIEW OF BIOPHYSICS AND BIOMOLECULAR STRUCTURE | 1999年 / 28卷

关键词：

analytical ultracentrifugation; thermodynamics; interacting systems; macromolecular characterization; hydrodynamics;

D O I：

10.1146/annurev.biophys.28.1.75

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Analytical ultracentrifugation is a classical method of biochemistry and molecular biology. Because it is a primary technique, sedimentation can provide first-principle hydrodynamic and first-principle thermodynamic information for nearly any molecule, in a wide range of solvents and over a wide range of solute concentrations. For many questions, it is the technique of choice. This review stresses what information is available from analytical ultracentrifugation and how that information is being extracted and used in contemporary applications.

引用

页码：75 / 100

页数：26

共 154 条

[1] CHEMICALLY REACTING SYSTEMS OF TYPE A + B-][-AB .I. SEDIMENTATION EQUILIBRIUM OF IDEAL SOLUTIONS [J].

ADAMS, ET .

ANNALS OF THE NEW YORK ACADEMY OF SCIENCES, 1969, 164 (A1) :226-&

[2]

ADAMS ET, 1994, J AM CHEM SOC, V86, P3454

[3]

[Anonymous], 1975, Foundations of ultracentrifugal analysis

[4]

ARAKAWA T, 1994, J BIOL CHEM, V269, P27833

[5]

ARAKAWA T, 1985, METHOD ENZYMOL, V117, P60

[6] SIMULTANEOUS DETERMINATION OF THE INDIVIDUAL CONCENTRATION GRADIENTS OF 2 SOLUTE SPECIES IN A CENTRIFUGED MIXTURE - APPLICATION TO ANALYTICAL ULTRACENTRIFUGATION [J].

ATTRI, AK ;

MINTON, AP .

ANALYTICAL BIOCHEMISTRY, 1987, 162 (02) :409-419

[7] TECHNIQUE AND APPARATUS FOR AUTOMATED FRACTIONATION OF THE CONTENTS OF SMALL CENTRIFUGE TUBES - APPLICATION TO ANALYTICAL ULTRACENTRIFUGATION [J].

ATTRI, AK ;

MINTON, AP .

ANALYTICAL BIOCHEMISTRY, 1986, 152 (02) :319-328

[8] PROTEIN PROTEIN INTERACTIONS OF HIV-1 REVERSE-TRANSCRIPTASE - IMPLICATION OF CENTRAL AND C-TERMINAL REGIONS IN SUBUNIT BINDING [J].

BECERRA, SP ;

KUMAR, A ;

LEWIS, MS ;

WIDEN, SG ;

ABBOTTS, J ;

KARAWYA, EM ;

HUGHES, SH ;

SHILOACH, J ;

WILSON, SH .

BIOCHEMISTRY, 1991, 30 (50) :11707-11719

[9] Molecular mass determination by sedimentation velocity experiments and direct fitting of the concentration profiles [J].

Behlke, J ;

Ristau, O .

BIOPHYSICAL JOURNAL, 1997, 72 (01) :428-434

[10] NUMERICAL SOLUTIONS OF LAMM EQUATION .4. ROTOR SLOWING EXPERIMENTS [J].

BILLICK, IH ;

DISHON, M ;

WEISS, GH ;

YPHANTIS, DA .

BIOPOLYMERS, 1967, 5 (10) :1021-&

← 1 2 3 4 5 6 7 8 9 10 →