Rapid discrimination of cytK-1 and cytK-2 genes in Bacillus cereus strains by a novel duplex PCR system

被引：76

作者：

Guinebretiere, MH

Fagerlund, A

Granum, PE

Nguyen-The, C

机构：

[1] INRA, UMR A408, Secur & Qual Prod Origine Vegetale, F-84914 Avignon 9, France

[2] Norwegian Sch Vet Sci, Dept Food Safety & Infect Biol, Oslo, Norway

来源：

FEMS MICROBIOLOGY LETTERS | 2006年 / 259卷 / 01期

关键词：

Bacillus cereus; cytotoxin K; cytK-1; gene; cytK-2; diarrhoeal food poisoning; PCR; detection;

D O I：

10.1111/j.1574-6968.2006.00247.x

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Bacillus cereus is the causative agent of gastrointestinal diarrhoea. At least three known enterotoxins may be involved in this syndrome: nonhaemolytic (Nhe) enterotoxin, Hbl enterotoxin and cytotoxin K. Two different forms were recently described for cytotoxin K, encoded by cytK-1 and cytK-2 genes. The CytK-1 toxin appeared to carry a high toxicity, but there is currently no method available to rapidly detect and discriminate the B. cereus strains able to produce this CytK-1 form. In this study, a duplex PCR assay was developed and validated on 162 known cytotoxin-containing strains. This PCR method is the first molecular tool to provide rapid detection and discrimination of cytK-1- and cytK-2-carrying B. cereus strains.

引用

页码：74 / 80

页数：7

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