Genetic analysis of calmodulin and its targets in Saccharomyces cerevisiae

被引:144
作者
Cyert, MS [1 ]
机构
[1] Stanford Univ, Dept Sci Biol, Stanford, CA 94305 USA
关键词
calcium; signal transduction; calcineurin; myosin; spindle pole body;
D O I
10.1146/annurev.genet.35.102401.091302
中图分类号
Q3 [遗传学];
学科分类号
071007 ; 090102 ;
摘要
Calmodulin, a small, ubiquitous Ca2+-binding protein, regulates a wide variety of proteins and processes in all eukaryotes. CMD1, the single gene encoding calmodulin in S. cerevisiae, is essential, and this review discusses studies that identified many of calmodulin's physiological targets and their functions in yeast cells. Calmodulin performs essential roles in mitosis, through its regulation of Nuf1p/Spc110p, a component of the spindle pole body, and in bud growth, by binding Myo2p, an unconventional class V myosin required for polarized secretion. Surprisingly, mutant calmodulins that fail to bind Ca2+ can perform these essential functions. Calmodulin is also required for endocytosis in yeast and participates in Ca2+-dependent, stress-activated signaling pathways through its regulation of a protein phosphatase, calcineurin, and the protein kinases, Cmk1p and Cmk2p. Thus, calmodulin performs important physiological functions in yeast cells in both its Ca2+-bound and Ca2+-free form.
引用
收藏
页码:647 / 672
页数:26
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