Cytoplasmic and serum galectin-3 in diagnosis of thyroid malignancies

被引：43

作者：

Inohara, Hidenori ^{[1
]}

Segawa, Tatsuya ^{[2
]}

Miyauchi, Akira ^{[3
]}

Yoshii, Tadashi ^{[1
]}

Nakahara, Susumu ^{[1
]}

Raz, Avraham ^{[4
]}

Maeda, Masahiro ^{[2
]}

Miyoshi, Eiji ^{[5
]}

Kinoshita, Noriaki ^{[2
]}

Yoshida, Hiroshi ^{[3
]}

Furukawa, Masashi ^{[1
]}

Takenaka, Yukinori ^{[1
]}

Takamura, Yuki ^{[3
]}

Ito, Yasuhiro ^{[3
]}

Taniguchi, Naoyuki ^{[6
]}

机构：

[1] Osaka Univ, Dept Otolaryngol, Sch Med, Suita, Osaka 5650871, Japan

[2] Immunobiol Labs IBL Co Ltd, Gunma, Japan

[3] Kuma Hosp, Kobe, Hyogo, Japan

[4] Karmanos Canc Inst, Tumor Progress & Metastasis Program, Detroit, MI USA

[5] Osaka Univ, Sch Med, Dept Mol Biochem & Clin Invest, Osaka, Japan

[6] Seikagaku Corp, Res Inst Microbial Dis, Dept Dis Glyc, Osaka, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 376卷 / 03期

关键词：

Enzyme-linked immunosorbent assay; Galectin-3; Thyroid; Carcinoma;

D O I：

10.1016/j.bbrc.2008.09.041

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

in order to address whether galectin-3 in the sera and fine needle aspirates serve as a diagnostic marker distinguishing between benign and malignant thyroid nodules, we developed an enzyme-linked immunosorbent assay. We quantified galectin-3 in fine needle aspirates from a series of I IS patients with thyroid nodules and serum galectin-3 from another series of 46 patients, which were compared with final histology after thyroidectomy. Relative galectin-3 value (ng/mg), defined as galectin-3 concentration (ng/ml) divided by total protein concentration (mg/ml) in fine needle aspirates, was significantly higher in papillary carcinoma than in the other thyroid entities. There was no significant difference in serum galectin-3 level among patients with thyroid nodules and healthy individuals. Accordingly, relative galectin-3 value allows a definitive diagnosis of papillary carcinoma independent of cellular morphology, whereas serum galectin-3 does not serve as a marker for papillary carcinoma. (C) 2008 Elsevier Inc. All rights reserved.

引用

页码：605 / 610

页数：6

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