Rapid and reliable method of extracting DNA and RNA from sweetpotato, Ipomoea batatas (L). Lam

被引：123

作者：

Kim, SH ^{[1
]}

Hamada, T ^{[1
]}

机构：

[1] Ishikawa Prefectural Univ, Res Inst Bioresource & Biotechnol, Nonoichi, Ishikawa 9218836, Japan

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 23-24期

关键词：

DNA; RNA; Sweetpotato (Ipomoea batatas (L.) Lam.);

D O I：

10.1007/s10529-005-3891-2

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A quick, simple and reliable method of extracting DNA from sweetpotato (Ipomoea batatas (L.) Lam.) has been developed. The method was applied successfully for extraction of total DNA from leaves and total RNA from leaves and various tissues. The yield of DNA extracted by this procedure was high (about 1 mg/g leaf tissue). The extracted DNA was completely digested by restriction endonucleases indicating the absence of common contaminating compounds. The absorbancy ratios of A260/A230 and A260/A280 of isolated RNA were approx. 2 and the yield was about 0.2 mg/g fresh wt. CIPK and tublin genes were successfully amplified by RT-PCR, suggesting the integrity of isolated RNA. The total DNA and RNA isolated by this method was of sufficient quality for subsequent molecular analysis.

引用

页码：1841 / 1845

页数：5

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