Estrogenic effects of two derivatives of icariin on human breast cancer MCF-7 cells

被引:94
作者
Ye, HY [1 ]
Lou, YJ [1 ]
机构
[1] Zhejiang Univ, Coll Pharmaceut Sci, Dept Pharmacol & Toxicol, Hangzhou 310027, Peoples R China
基金
中国国家自然科学基金;
关键词
icariin; icaritin; desmethylicaritin; MCF-7; cells; phytoestrogens;
D O I
10.1016/j.phymed.2004.10.002
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The aims of the present study were to determine the estrogenic activities of icariin (ICA) and its derivatives and their structure-estrogenic activity relationship. Therefore, icaritin (ICT) and desmethylicaritin (DICT) were derived from ICA. The estrogenic activities of ICA, ICT and DICT were examined by cell proliferation and progestogen receptor mRNA expression of estrogen-receptor-positive MCF-7 cells. Current studies exhibited that ICT and DICT both markedly enhanced the proliferation of MCF-7 cells; as compared to estradiol (100%), their relative proliferative effects (RPE) were 90% and 94%, respectively. Cell proliferation induced by ICT and DICT was completely antagonized by ICI182,780. ICT and DICT increased progestogen receptor (PR) at mRNA levels at 48 h after treatment, although the effects were not as prominent as 17 beta-estradiol (E-2). These phenomena were not observed with ICA. Results demonstrate that ICT and DICT (nonconjugated forms) possess estrogen-like activity; however, ICA appears to have no estrogenicity in the MCF-7 cell line model in vitro. (c) 2005 Elsevier GmbH. All rights reserved.
引用
收藏
页码:735 / 741
页数:7
相关论文
共 34 条
[1]  
BAIL JC, 1998, CANC LETT, V130, P209
[2]   Phyto-oestrogens: Where are we now? [J].
Bingham, SA ;
Atkinson, C ;
Liggins, J ;
Bluck, L ;
Coward, A .
BRITISH JOURNAL OF NUTRITION, 1998, 79 (05) :393-406
[3]  
CARMICHAEL J, 1987, CANCER RES, V47, P943
[4]  
Feng D, 2001, CHINA PHARM, V12, P426
[5]  
GAU S, 1999, CHINA J CHINESE MAT, V24, P249
[6]  
Guang LX, 1996, CHINESE PHARMACOL B, V12, P320
[7]  
Jiang Yong-nan, 2002, Zhongguo Zhongyao Zazhi, V27, P221
[8]   METABOLITES OF DIETARY (SOYA) ISOFLAVONES IN HUMAN URINE [J].
KELLY, GE ;
NELSON, C ;
WARING, MA ;
JOANNOU, GE ;
REEDER, AY .
CLINICA CHIMICA ACTA, 1993, 223 (1-2) :9-22
[9]   Flavonoids from Epimedium wanshanense [J].
Li, WK ;
Zhang, RY ;
Xiao, PG .
PHYTOCHEMISTRY, 1996, 43 (02) :527-530
[10]  
Liang Hairui, 1993, China Journal of Chinese Materia Medica, V18, P677