Structure of a Type IV Secretion System Core Complex

被引：238

作者：

Fronzes, Remi ^{[1
]}

Schafer, Eva ^{[1
]}

Wang, Luchun ^{[1
]}

Saibil, Helen R. ^{[1
]}

Orlova, Elena V. ^{[1
]}

Waksman, Gabriel ^{[1
,2
]}

机构：

[1] Univ London Birkbeck Coll, Inst Struct & Mol Biol, Sch Crystallog, London WC1E 7HX, England

[2] UCL, Div Biosci, London WC1E 6BT, England

来源：

SCIENCE | 2009年 / 323卷 / 5911期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

AGROBACTERIUM-TUMEFACIENS; PROTEIN SUBASSEMBLIES; ESCHERICHIA-COLI; VIRB9; DNA; BIOGENESIS; MACHINERY; SUBSTRATE; MEMBRANES; COMPONENT;

D O I：

10.1126/science.1166101

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Type IV secretion systems (T4SSs) are important virulence factors used by Gram- negative bacterial pathogens to inject effectors into host cells or to spread plasmids harboring antibiotic resistance genes. We report the 15 angstrom resolution cryo- electron microscopy structure of the core complex of a T4SS. The core complex is composed of three proteins, each present in 14 copies and forming a similar to 1.1- megadalton two-chambered, double membrane- spanning channel. The structure is double- walled, with each component apparently spanning a large part of the channel. The complex is open on the cytoplasmic side and constricted on the extracellular side. Overall, the T4SS core complex structure is different in both architecture and composition from the other known double membrane- spanning secretion system that has been structurally characterized.

引用

页码：266 / 268

页数：3

共 17 条

[1] The lipoprotein VirB7 interacts with VirB9 in the membranes of Agrobacterium tumefaciens [J].

Baron, C ;

Thorstenson, YR ;

Zambryski, PC .

JOURNAL OF BACTERIOLOGY, 1997, 179 (04) :1211-1218

[2] NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system [J].

Bayliss, Richard ;

Harris, Richard ;

Coutte, Loic ;

Monier, Amy ;

Fronzes, Remi ;

Christie, Peter J. ;

Driscoll, Paul C. ;

Waksman, Gabriel .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (05) :1673-1678

[3] Agrobacterium VirB10, an ATP energy sensor required for type IV secretion [J].

Cascales, E ;

Christie, PJ .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (49) :17228-17233

[4] Definition of a bacterial type IV secretion pathway for a DNA substrate [J].

Cascales, E ;

Christie, PJ .

SCIENCE, 2004, 304 (5674) :1170-1173

[5] Biogenesis, architecture, and function of bacterial type IV secretion systems [J].

Christie, PJ ;

Atmakuri, K ;

Krishnamoorthy, V ;

Jakubowski, S ;

Cascales, E .

ANNUAL REVIEW OF MICROBIOLOGY, 2005, 59 :451-485

[6] The 3D structure of a periplasm-spanning platform required for assembly of group 1 capsular polysaccharides in Escherichia coli [J].

Collins, Richard F. ;

Beis, Konstantinos ;

Dong, Changjiang ;

Botting, Catherine H. ;

McDonnell, Catherine ;

Ford, Robert C. ;

Clarke, Bradley R. ;

Whitfield, Chris ;

Naismith, James H. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (07) :2390-2395

[7] The Agrobacterium T-DNA transport pore proteins VirB8, VirB9, and VirB10 interact with one another [J].

Das, A ;

Xie, YH .

JOURNAL OF BACTERIOLOGY, 2000, 182 (03) :758-763

[8] The Agrobacterium tumefaciens virB7 gene product, a proposed component of the T-complex transport apparatus, is a membrane-associated lipoprotein exposed at the periplasmic surface [J].

Fernandez, D ;

Dang, TAT ;

Spudich, GM ;

Zhou, XR ;

Berger, BR ;

Christie, PJ .

JOURNAL OF BACTERIOLOGY, 1996, 178 (11) :3156-3167

[9] Evidence that F-plasmid proteins TraV, TraK and TraB assemble. into an envelope-spanning structure in Escherichia coli [J].

Harris, RL ;

Hombs, V ;

Silverman, PM .

MOLECULAR MICROBIOLOGY, 2001, 42 (03) :757-766

[10] Agrobacterium tumefaciens VirB9, an outer-membrane-associated component of a type IV secretion system, regulates substrate selection and T-pilus biogenesis [J].

Jakubowski, SJ ;

Cascales, E ;

Krishnamoorthy, V ;

Christie, PJ .

JOURNAL OF BACTERIOLOGY, 2005, 187 (10) :3486-3495

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