Bacteriostatic effects of hyaluronic acid

被引:182
作者
Pirnazar, P
Wolinsky, L
Nachnani, S
Haake, S
Pilloni, A
Bernard, GW [1 ]
机构
[1] Univ Calif Los Angeles, Ctr Hlth Sci 63 050, Dept Oral Biol, Sch Dent,Sect Oral Biol, Los Angeles, CA 90024 USA
[2] Univ Calif Los Angeles, Sch Dent, Sect Periodont, Los Angeles, CA 90024 USA
[3] Univ Calif Los Angeles, Sch Med, Dept Neurobiol, Los Angeles, CA 90024 USA
关键词
hyaluronic acid; therapeutic use; periodontal regeneration; bacteriostatic agents;
D O I
10.1902/jop.1999.70.4.370
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
Background: This investigation is one of a series of projects seeking to ascertain whether hyaluronic acid (HA) is therapeutically effective in tissue regeneration procedures. The rationale for these investigations is to test the hypothesis that HA can serve as a bioabsorbable carrier for other substrates as well as itself actively promote the regeneration of tissue. Methods: In this paper, we report on the bacteriostatic and bactericidal properties of 3 molecular weight formulations of recombinant HA (low, 141 kD; medium, 757 kD; and high, 1,300 kD) on selected oral and non-oral microorganisms in the planktonic phase. Three concentrations of each HA formulation were screened, 0.5, 1.0, and 2.0 mg/ml, using a standard broth culture assay. Results: Recombinant HA exerted varied bacteriostatic effects on all the bacterial strains tested depending on its molecular weight (MW) and concentration, The high concentrations of the medium MW HA had the greatest bacteriostatic effect, particularly on the Actinobacillus actinomycetemcomitans, Prevotella oris, Staphylococcus aureus, and Propionibacterium acnes strains. The 1.0 mg/ml concentration of high MW HA had the greatest overall bacteriostatic effect, inhibiting the growth of all 6 bacterial strains tested. Among the bacterial strains studied, HA was found to have no bactericidal effects, regardless of concentration or molecular weight. Conclusions: The results of this study suggest that HA in the MW range of 1,300 kD may prove beneficial in minimizing bacterial contamination of surgical wounds when used in guided tissue regeneration surgery.
引用
收藏
页码:370 / 374
页数:5
相关论文
共 13 条
[1]  
BARON EJ, 1990, DIAGNOSTIC MICROBIOL, P422
[2]   THE OSTEOGENIC STIMULATING EFFECT OF NEUROACTIVE CALCITONIN GENE-RELATED PEPTIDE [J].
BERNARD, GW ;
SHIH, C .
PEPTIDES, 1990, 11 (04) :625-632
[3]   BINDING AND INTERNALIZATION OF HYALURONATE BY HUMAN CUTANEOUS FIBROBLASTS [J].
BERTOLAMI, CN ;
BERG, S ;
MESSADI, DV .
MATRIX, 1992, 12 (01) :11-21
[4]  
BERTOLAMI CN, 1984, SOFT HARD TISSUE REP, P67
[5]  
GHOSH P, 1994, CLIN EXP RHEUMATOL, V12, P75
[6]   EVIDENCE FOR THE ABSENCE OF HYALURONIDASE ACTIVITY IN PORPHYROMONAS-GINGIVALIS [J].
GRENIER, D ;
MICHAUD, J .
JOURNAL OF CLINICAL MICROBIOLOGY, 1993, 31 (07) :1913-1915
[7]  
MOMBELLI A, 1995, J CLIN PERIODONTOL, V22, P124
[8]   MICROORGANISMS IN POLYTETRAFLUOROETHYLENE BARRIER MEMBRANES FOR GUIDED TISSUE REGENERATION [J].
NOWZARI, H ;
SLOTS, J .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1994, 21 (03) :203-210
[9]  
PILLONI A, 1996, THESIS UCLA DEP OR B
[10]   NEW ATTACHMENT AND BONE-FORMATION IN PERIODONTAL DEFECTS FOLLOWING TREATMENT OF SUBMERGED ROOTS WITH GUIDED TISSUE REGENERATION [J].
SANDER, L ;
KARRING, T .
JOURNAL OF CLINICAL PERIODONTOLOGY, 1995, 22 (04) :295-299