N-terminal labeling of proteins by the Pictet-Spengler reaction

被引：39

作者：

Sasaki, Tsubasa ^{[1
]}

Kodama, Koichiro ^{[1
]}

Suzuki, Hiroaki ^{[1
]}

Fukuzawa, Seketsu ^{[1
]}

Tachibana, Kazuo ^{[1
]}

机构：

[1] Univ Tokyo, Dept Chem, Sch Sci, Bunkyo Ku, Tokyo 1130033, Japan

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 2008年 / 18卷 / 16期

关键词：

Pictet-Spengler reaction; myoglobin N-terminal; biotin; transamination reaction; keto aldehyde;

D O I：

10.1016/j.bmcl.2008.07.033

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

The Pictet-Spengler reaction was applied to the N-terminal labeling of horse heart myoglobin. This was performed in the following two steps: (1) conversion of the N-terminal glycine residue to an alpha-keto aldehyde by a transamination reaction and (2) condensation of the resulting activated myoglobin with tryptamine analogues by the Pictet-Spengler reaction. Ultraviolet (UV)/visible (vis) absorption and circular dichroism (CD) spectral data revealed that the tertiary structure of myoglobin was not altered by the Pictet-Spengler reaction. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：4550 / 4553

页数：4

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