Comparison of responses to electrical stimulation and whisker deflection using two different voltage-sensitive dyes in mouse barrel cortex in vivo

被引:23
作者
Civillico, EF [1 ]
Contreras, D [1 ]
机构
[1] Univ Penn, Sch Med, Dept Neurosci, Philadelphia, PA 19104 USA
关键词
VSD; fluorescence; imaging; RH795; RH1691;
D O I
10.1007/s00232-005-0828-6
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
We examined the spatial structure of noise in optical recordings made with two commonly used voltage-sensitive dyes (RH795 and RH1691) in mouse barrel cortex in vivo, and determined that the signal-to-noise ratio of the two dyes was comparable when averaging over barrel-sized areas, or at single pixels distant from large blood vessels. We examined the spatiotemporal development of whisker- and electrically-evoked optical responses by quantifying the area of activated cortical surface as a function of time. Whisker and electrical stimuli activated cortical areas between 0.2-2.0 mm(2) depending on intensity. More importantly, both types of activation recruited cortical area at similar rates and showed a linear relationship between the maximal activated area and the peak rate of increase of the activated area. We propose a general rule of supragranular cortical activation in which the initial spreading speed of the response determines the total activated area, independent of the type of activation. Finally, despite comparable single-response kinetics, we observed greater paired-pulse depression of whisker-evoked responses relative to electrically-evoked responses.
引用
收藏
页码:171 / 182
页数:12
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