Analysis of the heat shock response of Neisseria meningitidis with cDNA- and oligonucleotide-based DNA microarrays

被引:33
作者
Guckenberger, M
Kurz, S
Aepinus, C
Theiss, S
Haller, S
Leimbach, T
Panzner, U
Weber, J
Paul, H
Unkmeir, A
Frosch, M
Dietrich, G
机构
[1] Univ Wurzburg, Inst Hyg & Microbiol, D-97080 Wurzburg, Germany
[2] MWG Biotech AG, D-85560 Ebersberg, Germany
关键词
D O I
10.1128/JB.184.9.2546-2551.2002
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Oligonucleotide- and cDNA-based microarrays comprising a subset of Neisseria meningitidis genes were assessed for study of the meningococcal heat shock response and found to be highly suitable for transcriptional profiling of N. meningitidis. Employing oligonucleotide arrays encompassing the entire genome of N. meningitidis, we analyzed the meningococcal heat shock response on a global scale and identified 55 heat shock-deregulated open reading frames (34 induced and 21 repressed).
引用
收藏
页码:2546 / 2551
页数:6
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