Developmental expression of thrombin in zebrafish embryos: A novel model to study hemostasis

被引:18
作者
Jagadeeswaran, P
Liu, YC
机构
[1] Dept. of Cell. and Struct. Biology, Univ. of Texas Hlth. Sci. Ctr. at S., San Antonio, TX 78284-7762
关键词
thrombin; cDNA sequence; zebrafish; hemostasis; embryogenesis;
D O I
10.1006/bcmd.1997.0132
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A partial cDNA encoding zebrafish prothrombin has been cloned and used as a probe to study the temporal expression of prothrombin mRNA during early embryonic development, The results revealed accumulation of prothrombin mRNA in diverse tissues such as the eyes and myotomes in early embryogenesis, We have also examined the enzymatic activity of thrombin in converting fibrinogen to fibrin in individual embryos at different stages of development, We found that the fibrin-forming activity does not temporally correlate with the first presence of thrombin mRNA in the early stages of embryogenesis, but does correlate with the initiation of blood formation, Our ability to observe the fibrin-forming activity in single individual embryo will facilitate studies on identifying recessive mutations affecting blood coagulation, such as the regulatory gene mutations controlling the clotting factor genes, Furthermore, the observation of thrombin activity will also facilitate studies on the blood coagulation pathways in the early embryogenesis in this zebrafish model.
引用
收藏
页码:147 / 156
页数:10
相关论文
共 19 条
  • [1] PARTIAL CHARACTERIZATION OF VERTEBRATE PROTHROMBIN CDNAS - AMPLIFICATION AND SEQUENCE-ANALYSIS OF THE B-CHAIN OF THROMBIN FROM 9 DIFFERENT SPECIES
    BANFIELD, DK
    MACGILLIVRAY, RTA
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1992, 89 (07) : 2779 - 2783
  • [2] Characterization of a membrane protease from rat submaxillary-gland mitochondria that possesses thrombin-like activity
    Bharadwaj, M
    Bharadwaj, D
    Hathi, RN
    [J]. BIOCHEMICAL JOURNAL, 1996, 313 : 193 - 199
  • [3] AGE-SPECIFIC REGULATION OF CLOTTING FACTOR-IX GENE-EXPRESSION IN NORMAL AND TRANSGENIC MICE
    BOLAND, EJ
    LIU, YC
    WALTER, CA
    HERBERT, DC
    WEAKER, FJ
    ODOM, MW
    JAGADEESWARAN, P
    [J]. BLOOD, 1995, 86 (06) : 2198 - 2205
  • [4] RECIPROCAL MODULATION OF ASTROCYTE STELLATION BY THROMBIN AND PROTEASE NEXIN-1
    CAVANAUGH, KP
    GURWITZ, D
    CUNNINGHAM, DD
    BRADSHAW, RA
    [J]. JOURNAL OF NEUROCHEMISTRY, 1990, 54 (05) : 1735 - 1743
  • [5] MITOGENIC ACTIVITY OF BLOOD COMPONENTS .1. THROMBIN AND PROTHROMBIN
    CHEN, LB
    BUCHANAN, JM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1975, 72 (01) : 131 - 135
  • [6] Role of the thrombin receptor In development and evidence for a second receptor
    Connolly, AJ
    Ishihara, H
    Kahn, ML
    Farese, RV
    Coughlin, SR
    [J]. NATURE, 1996, 381 (6582) : 516 - 519
  • [7] DOOLITTLE RF, 1993, THROMB HAEMOSTASIS, V70, P24
  • [8] The zebrafish: Heritable disorders in transparent embryos
    Driever, W
    Fishman, MC
    [J]. JOURNAL OF CLINICAL INVESTIGATION, 1996, 97 (08) : 1788 - 1794
  • [9] BLOOD-COAGULATION
    JACKSON, CM
    NEMERSON, Y
    [J]. ANNUAL REVIEW OF BIOCHEMISTRY, 1980, 49 : 765 - 811
  • [10] A hemophilia model in zebrafish: Analysis of hemostasis
    Jagadeeswaran, P
    Liu, YC
    [J]. BLOOD CELLS MOLECULES AND DISEASES, 1997, 23 (03) : 52 - 57