Dynamic light-scattering analysis of the electrostatic interaction of hexahistidine-tagged cytochrome P450 enzyme with semiconductor quantum dots

被引：68

作者：

Ipe, Binil Itty ^{[1
]}

Shukla, Anuj ^{[1
]}

Lu, Huachang ^{[1
]}

Zou, Bo ^{[1
]}

Rehage, Heinz ^{[1
]}

Niemeyer, Christof M. ^{[1
]}

机构：

[1] Univ Dortmund, Fachbereich Chem, D-44227 Dortmund, Germany

来源：

CHEMPHYSCHEM | 2006年 / 7卷 / 05期

关键词：

D O I：

10.1002/cphc.200500660

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

Currently, there is great interest in the development of methods suitable for determining the stoichiometry of biomolecules attached to nanoparticles. We describe the use of the dynamic light-scattering technique (DLS) to determine the stoichiometry of the protein cytochrome P450(BS beta) attached to CdS and CdSe quantum dots (QDs). The enzyme-conjugated (DLS) have different diffusion kcharacteristics compared to the QD and enzyme precursors, expressed in their size, scattering intensity as well as zeto-potential values. The significant enhancement of the scattering intensity of QDs observed upon conjugation with the P450(BS beta) due to the refractive-index increment and the systematic variation in zeta potential resulting from charge neutralization of the anionic QDs by the cationic histidine-tagged P450(BS beta) have been used for stoichiometry determination.

引用

页码：1112 / 1118

页数：7

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