Streptococcus pneumoniae-induced regulation of cyclooxygenase-2 in human lung tissue

被引：46

作者：

Szymanski, Kolja V. ^{[1
]}

Toennies, Mario ^{[2
]}

Becher, Anne ^{[1
]}

Fatykhova, Diana ^{[1
]}

N'Guessan, Philippe D. ^{[1
]}

Gutbier, Birgitt ^{[1
]}

Klauschen, Frederick ^{[3
]}

Neuschaefer-Rube, Frank ^{[7
]}

Schneider, Paul ^{[4
]}

Rueckert, Jens ^{[5
]}

Neudecker, Jens ^{[5
]}

Bauer, Torsten T. ^{[2
]}

Dalhoff, Klaus ^{[8
]}

Droemann, Daniel ^{[8
]}

Gruber, Achim D. ^{[6
]}

Kershaw, Olivia ^{[6
]}

Temmesfeld-Wollbrueck, Bettina ^{[1
]}

Suttorp, Norbert ^{[1
]}

Hippenstiel, Stefan ^{[1
]}

Hocke, Andreas C. ^{[1
]}

机构：

[1] Charite, Dept Internal Med Infect Dis & Pulm Med, D-10117 Berlin, Germany

[2] HELIOS Klinikum Emil von Behring, Lungenklin Heckeshorn, Berlin, Germany

[3] Charite, Inst Pathol, D-10117 Berlin, Germany

[4] DRK Clin, Dept Gen & Thorac Surg, Berlin, Germany

[5] Charite, Dept Gen Visceral Vasc & Thorac Surg, D-10117 Berlin, Germany

[6] Free Univ Berlin, Dept Vet Pathol, Berlin, Germany

[7] Univ Potsdam, Inst Nutr Sci, Nuthetal, Germany

[8] Univ Hosp Schleswig Holstein, Med Clin 3, Lubeck, Germany

来源：

EUROPEAN RESPIRATORY JOURNAL | 2012年 / 40卷 / 06期

关键词：

Alveolar epithelial cells; cytokines; inflammation; lung infection; pneumonia; prostaglandins; B-DEPENDENT COX-2; COMMUNITY-ACQUIRED PNEUMONIA; PROSTAGLANDIN E-2; EPITHELIAL-CELLS; EXPRESSION; INFECTION; RECEPTOR; RELEASE; INHIBITION; ACTIVATION;

D O I：

10.1183/09031936.00186911

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

The majority of cases of community-acquired pneumonia are caused by Streptococcus pneumoniae and most studies on pneumococcal host interaction are based on cell culture or animal experiments. Thus, little is known about infections in human lung tissue. Cyclooxygenase-2 and its metabolites play an important regulatory role in lung inflammation. Therefore, we established a pneumococcal infection model on human lung tissue demonstrating mitogen-activated protein kinase (MAPK)-dependent induction of cyclooxygenase-2 and its related metabolites. In addition to alveolar macrophages and the vascular endothelium, cyclooxygenase-2 was upregulated in alveolar type II but not type I epithelial cells, which was confirmed in lungs of patients suffering from acute pneumonia. Moreover, we demonstrated the expression profile of all four E prostanoid receptors at the mRNA level and showed functionality of the E prostanoid(4) receptor by cyclic adenosine monophosphate production. Additionally, in comparison to previous studies, cyclooxygenase-2/prostaglandin E-2 related pro- and anti-inflammatory mediator regulation was partly confirmed in human lung tissue after pneumococcal infection. Overall, cell type-specific and MAPK-dependent cyclooxygenase-2 expression and prostaglandin E-2 formation in human lung tissue may play an important role in the early phase of pneumococcal infections.

引用

页码：1458 / 1467

页数：10

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