Phylogenetic analysis of a novel molecular isolate of spotted fever group rickettsiae from northern Peru -: Candidatus rickettsia andeanae

被引：60

作者：

Jiang, Ju

Blair, Patrick J.

Felices, Vidal

Moron, Cecilia

Cespedes, Manuel

Anaya, Elizabeth

Schoeler, George B.

Sumner, John W.

Olson, James G.

Richards, Allen L.

机构：

[1] USN, Med Res Ctr, Rickettsial Dis Dept, Silver Spring, MD 20910 USA

[2] Henry M Jackson Fdn, Rockville, MD 20852 USA

[3] Naval Med Res Ctr Detachment, Lima, Peru

[4] Minist Hlth, Natl Inst Hlth, Lima, Peru

[5] Ctr Dis Control & Prevent, Atlanta, GA 30033 USA

[6] Uniformed Serv Univ Hlth Sci, Bethesda, MD 20814 USA

来源：

RICKETTSIOSES: FROM GENOME TO PROTEOME, PATHOBIOLOGY, AND RICKETTSIAE AS AN INTERNATIONAL THREAT | 2005年 / 1063卷

关键词：

Peru; ticks; spotted fever rickettsiae; phylogenetic analysis;

D O I：

10.1196/annals.1355.054

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 [免疫学];

摘要：

Phylogenetic analysis of five rickettsial genes (17-kDa gene, gltA, ompB, ompA, and sca4) from two molecular isolates of Candidatus Rickettsia andeanae from two ticks (Amblyomma maculatum and Ixodes boliviensis) collected from two domestic horses living in two separate locations in northern Peru (Coletas and Naranjo) was conducted to more clearly characterize this recently reported novel spotted fever group (SFG) rickettsia. Following nested polymerase chain reaction (PCR) amplification of the 17-kDa gene, gltA, ompB, ompA, and sca4, amplicons were purified, sequenced, and compared to those downloaded from GenBank. Phylogenetic analyses of the Candidatus Rickettsia andeanae sequences generated from 17-kDa gene (483 bp), gltA (1185 bp), ompA (1598 lip), ompB (4839 bp), and sca4 (2634 bp) demonstrated that they aligned strongly with those of SFG rickettsiae. Moreover, the sequences of these five genes most closely aligned with the following rickettsiae: ompA: Rickettsia sp RpA4 (98.03%), R. sp DnS28 (97.90%), and R. rhipicephali and R. massiliae (97.11%); ompB: R. aeschlimannii (97.22%), R. rhipicephali (97.20%), and R. sp Bar 29 (97.10%); and sca4: R. massiliae (97.8%), R. rhipicephali, and R. slovaca (97.7%). These results from the additional phylogenetic analyses of Candidatus Rickettsia andeanae confirm its inclusion within, and distance and uniqueness from, other known SFG rickettsiae.

引用

页码：337 / 342

页数：6

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