LRP-1/TβR-V mediates TGF-β1-induced growth inhibition in CHO cells

被引：47

作者：

Tseng, WF

Huang, SS

Huang, JS

机构：

[1] St Louis Univ, Sch Med, Dept Biochem & Mol Biol, St Louis, MO 63104 USA

[2] Natl Def Med Ctr, Inst Life Sci, Taipei, Taiwan

来源：

FEBS LETTERS | 2004年 / 562卷 / 1-3期

关键词：

transforming growth factor-beta 1; growth inhibition; type V transforming growth factor-beta receptor; lipoprotein receptor-related protein-1; CHO cell; lipoprotein receptor-related protein-1minireceptor;

D O I：

10.1016/S0014-5793(04)00185-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The type V transforming growth factor-beta (TGF-beta) receptor (TbetaR-V) is hypothesized to be involved in cellular growth inhibition by TGF-beta(1). Recently, TbetaR-V was found to be identical to low density lipoprotein receptor-related protein-1 (LRP-1). Here we demonstrate that TGF-beta(1), inhibits growth of wild-type CHO cells but not LRP-1-deficient mutant cells (CHO-LRP-1(-) cells). Stable transfection of CHO-LRP-1(-) cells with LRP-1 cDNA restores the wild-type morphology and the sensitivity to growth inhibition by TGF-beta(1). In addition, overexpression of LRP-1 minireceptors exerts a dominant negative effect and attenuates the growth inhibitory response to TGF-beta(1) in wild-type CHO cells. These results suggest that LRP-1/TbetaR-V is critical for TGF-beta(1)-mediated growth inhibition in CHO cells. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

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页码：71 / 78

页数：8

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