Photoelectric measurements of self-assembled and supported planar lipid bilayers: a new technique for studying apoptosis

被引：7

作者：

Feng, J ^{[1
]}

Ci, YX

Zhang, CY

Ottova, AL

Tien, HT

机构：

[1] Peking Univ, Dept Chem, Beijing 100871, Peoples R China

[2] Slovak Univ Technol Bratislava, Ctr Interface Sci, Microelect Dept, Bratislava, Slovakia

[3] Michigan State Univ, Dept Physiol, E Lansing, MI 48824 USA

来源：

ELECTROCHEMISTRY COMMUNICATIONS | 1999年 / 1卷 / 3-4期

基金：

中国国家自然科学基金;

关键词：

apoptosis; bilayer lipid membranes; planar lipid bilayers; photoelectric effects; DNA; Taxol;

D O I：

10.1016/S1388-2481(99)00021-1

中图分类号：

O646 [电化学、电解、磁化学];

学科分类号：

081704 ;

摘要：

A new method based on photoelectrochemistry for analyzing apoptosis of bilayer lipid membranes (s-BLMs) containing MCF-7 nuclei is reported. The s-BLM cell responded to white light (200-800 nm). During the apoptosis induced by Taxol, the photoelectric current of the cell decreased, suggesting degradation of the nuclear DNA. Electron transfer along the DNA double helix and along the nuclear skeleton is assumed in the interpretation. This novel photoelectric analytical method may provide a rapid and sensitive technique to evaluate apoptosis. (C) 1999 Elsevier Science S.A. All rights reserved.

引用

页码：145 / 147

页数：3

共 22 条

[1]

BERTRAND R, 1991, CANCER RES, V51, P6280

[2] APOPTOSIS AND ITS MODULATION IN HUMAN PROMYELOCYTIC HL-60 CELLS TREATED WITH DNA TOPOISOMERASE-I AND TOPOISOMERASE-II INHIBITORS [J].

BERTRAND, R ;

SOLARY, E ;

JENKINS, J ;

POMMIER, Y .

EXPERIMENTAL CELL RESEARCH, 1993, 207 (02) :388-397

[3] Photoelectric behavior of mammalian cells and its bioanalytical applications [J].

Ci, YX ;

Zhang, CY ;

Feng, J .

BIOELECTROCHEMISTRY AND BIOENERGETICS, 1998, 45 (02) :247-251

[4]

CI YX, 1997, C WORLDW CHIN YOUNG, V12, P583

[5] PHOTOINDUCED ELECTRON-TRANSFER IN DNA MATRIX FROM INTERCALATED ETHIDIUM TO CONDENSED METHYLVIOLOGEN [J].

FROMHERZ, P ;

RIEGER, B .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1986, 108 (17) :5361-5362

[6] Oxidative DNA damage through long-range electron transfer [J].

Hall, DB ;

Holmlin, RE ;

Barton, JK .

NATURE, 1996, 382 (6593) :731-735

[7]

Kerr J.F.R., 1987, P93

[8] APOPTOSIS - BASIC BIOLOGICAL PHENOMENON WITH WIDE-RANGING IMPLICATIONS IN TISSUE KINETICS [J].

KERR, JFR ;

WYLLIE, AH ;

CURRIE, AR .

BRITISH JOURNAL OF CANCER, 1972, 26 (04) :239-+

[9] LONG-RANGE PHOTOINDUCED ELECTRON-TRANSFER THROUGH A DNA HELIX [J].

MURPHY, CJ ;

ARKIN, MR ;

JENKINS, Y ;

GHATLIA, ND ;

BOSSMANN, SH ;

TURRO, NJ ;

BARTON, JK .

SCIENCE, 1993, 262 (5136) :1025-1029

[10]

NEWMEYER DD, 1991, METHOD CELL BIOL, V36, P607

← 1 2 3 →