DNA double-strand breaks in mouse kidney cells with age

被引：45

作者：

Singh, NP

Ogburn, CE

Wolf, NS

van Belle, G

Martin, GM

机构：

[1] Univ Washington, Dept Bioengn, Seattle, WA 98195 USA

[2] Univ Washington, Dept Genet, Seattle, WA 98195 USA

[3] Univ Washington, Dept Pathol, Seattle, WA 98195 USA

[4] Univ Washington, Dept Biostat, Seattle, WA 98195 USA

[5] Univ Washington, Dept Environm Hlth, Seattle, WA 98195 USA

来源：

BIOGERONTOLOGY | 2001年 / 2卷 / 04期

关键词：

aging; Biojector; DNA double-strand breaks; kidney cells; neutral microgel electrophoresis; pathological lesions;

D O I：

10.1023/A:1013262327193

中图分类号：

R592 [老年病学]; C [社会科学总论];

学科分类号：

03 ; 0303 ; 100203 ;

摘要：

A Biojector device fitted with a CO2 cartridge was used to prepare single cell suspensions from kidneys of 12-month- (middle-aged) and 24-month-old (old) C57B1/6 mice. Microgel electrophoresis of DNA from these cells revealed a modest but significant 7.3% increase (P = 0.04) in DNA double-strand breaks in old mice. This increase is equivalent to the DNA damage induced by 0.1 Gray of X-rays (5 double-strand breaks) in kidney cells of 10-month-old mice, as determined by a standard calibration curve. Greater DNA damage with aging was also positively correlated with higher levels of pathology in the kidneys.

引用

页码：261 / 270

页数：10

共 51 条

[1] Thymine metabolism and thymineless death in prokaryotes and eukaryotes [J].