Analysis of gamma-hydroxybutyrate (GHB) in urine by gas chromatography-mass spectrometry

被引:58
作者
McCusker, RR
Paget-Wilkes, H
Chronister, CW
Goldberger, BA
ElSohly, MA
机构
[1] Univ Florida, Coll Med, Dept Pathol Immunol & Lab Med, Forens Toxicol Lab, Gainesville, FL 32610 USA
[2] ElSohly Labs Inc, Oxford, MS 38655 USA
关键词
D O I
10.1093/jat/23.5.301
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
A simple method for the direct analysis of gamma-hydroxybutyrate (GHB) from human urine is described. The method uses solid-phase extraction, liquid-liquid extraction, and silyl-derivatization, then gas chromatographic- mass spectrometric analysis using GHB-d6 as the internal standard. The method was linear from 5 to 500 mg/L, and coefficients of variation were less than 10%. Twenty-six urine specimens previously analyzed by an existing method were analyzed and yielded GHB concentrations ranging from 0 to 6100 mg/L; the results correlated between the two methods. Compared with existing methods, the method described here is superior because it is specific to GHB and can discriminate between GHB and gamma-butyrolactone.
引用
收藏
页码:301 / 305
页数:5
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