High-efficiency Semliki Forest virus-mediated transduction in bovine adrenal chromaffin cells

被引：22

作者：

Duncan, RR ^{[1
]}

Don-Wauchope, AC ^{[1
]}

Tapechum, S ^{[1
]}

Shipston, MJ ^{[1
]}

Chow, RH ^{[1
]}

Estibeiro, P ^{[1
]}

机构：

[1] Univ Edinburgh, Sch Med, Membrane Biol Grp, Edinburgh EH8 9XD, Midlothian, Scotland

来源：

BIOCHEMICAL JOURNAL | 1999年 / 342卷

基金：

英国惠康基金;

关键词：

acidic vesicle; confocal; double C2 protein beta-isoform; exocytosis; SFV;

D O I：

10.1042/0264-6021:3420497

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Adrenal chromaffin cells are commonly used in studies of exocytosis. Progress in characterizing the molecular mechanisms has been slow, because no simple, high-efficiency technique is available for introducing and expressing heterologous cDNA in chromaffin cells. Here we demonstrate that Semliki Forest virus (SFV) vectors allow high-efficiency expression of heterologous protein in chromaffin cells.

引用

页码：497 / 501

页数：5

共 17 条

[1] SEMLIKI FOREST VIRUS EXPRESSION SYSTEM - PRODUCTION OF CONDITIONALLY INFECTIOUS RECOMBINANT PARTICLES [J].

BERGLUND, P ;

SJOBERG, M ;

GAROFF, H ;

ATKINS, GJ ;

SHEAHAN, BJ ;

LILJESTROM, P .

BIO-TECHNOLOGY, 1993, 11 (08) :916-920

[2]

CICCIARONE V, 1997, FOCUS, V16, P94

[3]

Daly C, 1997, J NEUROSCI, V17, P2365

[4] CLEAVAGE OF STRUCTURAL PROTEINS DURING ASSEMBLY OF HEAD OF BACTERIOPHAGE-T4 [J].

LAEMMLI, UK .

NATURE, 1970, 227 (5259) :680-+

[5] A NEW GENERATION OF ANIMAL-CELL EXPRESSION VECTORS BASED ON THE SEMLIKI FOREST VIRUS REPLICON [J].

LILJESTROM, P ;

GAROFF, H .

BIO-TECHNOLOGY, 1991, 9 (12) :1356-1361

[6] PATCH-CLAMP TECHNIQUES FOR TIME-RESOLVED CAPACITANCE MEASUREMENTS IN SINGLE CELLS [J].

LINDAU, M ;

NEHER, E .

PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY, 1988, 411 (02) :137-146

[7]

Liu F, 1996, J NEUROCHEM, V67, P1457

[8]

MA WJ, 1992, J BIOL CHEM, V267, P22728

[9]

MOSS B, 1989, ANN NY ACAD SCI, V569, P86, DOI 10.1111/j.1749-6632.1989.tb27360.x

[10] NEW MAMMALIAN EXPRESSION VECTORS [J].

MOSS, B ;

ELROYSTEIN, O ;

MIZUKAMI, T ;

ALEXANDER, WA ;

FUERST, TR .

NATURE, 1990, 348 (6296) :91-92

← 1 2 →