Rapid Method for Multi-residue Determination of Avermectins in Bovine Liver Using High-performance Liquid Chromatography with Fluorescence Detection

被引：25

作者：

Wang, Hai ^{[1
,2
]}

Wang, Zijian ^{[1
]}

Liu, SuYing ^{[2
]}

Liu, Ziru ^{[3
]}

机构：

[1] Chinese Acad Sci, State Key Lab Environm Aquat Chem, Ecoenvironm Sci Res Ctr, Beijing 100085, Peoples R China

[2] China Anim Dis Control Ctr, Qual Control & Inspect Ctr Anim Prod, Minist Agr, Beijing 100026, Peoples R China

[3] Beijing Res Inst Chem Engn & Met, Beijing 101149, Peoples R China

来源：

BULLETIN OF ENVIRONMENTAL CONTAMINATION AND TOXICOLOGY | 2009年 / 82卷 / 04期

关键词：

Avermectins; Residue; Liver; Fluorescence;

D O I：

10.1007/s00128-009-9638-3

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

A simple method using high-performance liquid chromatography and fluorescence detection is presented for the simultaneous determination of the antiparasitic agents avermectins (abamectin, ivermectin and doramectin) in bovine liver. Samples were extracted using acetonitrile and cleaned up on an alumina SPE column and derivatized with trifluoroacetic anhydride. The derivatives were detected using fluorescence detector set at 365 nm excitation and 475 nm emission wavelengths. As the results, the limit of quantification for three avermectins was about 1 mu g/kg. Inter-assay showed the mean recoveries of avermectins in bovine liver ranged from 72% to 81%, with RSD less than 9.9%.

引用

页码：395 / 398

页数：4