Coimmobilization of urease and glutamate dehydrogenase in electrochemically prepared polypyrrole-polyvinyl sulfonate films

被引：40

作者：

Gambhir, A

Gerard, M

Mulchandani, AK

Malhotra, BD

机构：

[1] Natl Phys Lab, Biomol Elect & Conducting Polymer Res Grp, New Delhi 110012, India

[2] Univ Calif Riverside, Dept Environm Chem & Engn, Riverside, CA 92521 USA

来源：

APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY | 2001年 / 96卷 / 1-3期

关键词：

coimmobilization; urease; glutamate dehydrogenase; urea; biosensor;

D O I：

10.1385/ABAB:96:1-3:249

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Immobilization of urease and glutamate dehydrogenase enzymes in electrochemically prepared polypyrrole-polyvinyl sulfonate films (PPY-PVS) was carried out using physical adsorption and electrochemical entrapment techniques. Detailed studies on optimum pH, Fourier transform infrared spectroscopy, cyclic voltammetry, and scanning electron microscopy of the enzymes in the immobilized state were conducted. The value of the apparent Michaelis-Menten constant was experimentally determined to be 2.5 and 2.7 for physically adsorbed and electrochemically entrapped urease in PPY-PVS films, respectively.

引用

页码：249 / 257

页数：9

共 17 条

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