The amino-terminal region of major capsid protein P3 is essential for self-assembly of single-shelled core-like particles of Rice dwarf virus

被引:18
作者
Hagiwara, K
Higashi, T
Miyazaki, N
Naitow, H
Cheng, RH
Nakagawa, A
Mizuno, H
Tsukihara, T
Omura, T [1 ]
机构
[1] Natl Agr Res Ctr, Virol Lab, Tsukuba, Ibaraki 3058666, Japan
[2] Osaka Univ, Inst Prot Res, Suita, Osaka 5650871, Japan
[3] Natl Inst Agrobiol Sci, Tsukuba, Ibaraki 3058602, Japan
[4] Karolinska Inst, Dept Biosci Novum, S-14157 Huddinge, Sweden
关键词
D O I
10.1128/JVI.78.6.3145-3148.2004
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The core protein P3 of Rice dwarf virus constructs asymmetric dimers, one of which is inserted by the amino-terminal region of another P3 protein. The P3 proteins with serial amino-terminal deletions, expressed in a baculovirus system, formed particles with gradually decreasing stability. The capacity for self-assembly disappeared when 52 of the amino-terminal amino acids had been deleted. These results demonstrated that insertion of the amino-terminal arm of one P3 protein into another appears to play an important role in stabilizing the core particles.
引用
收藏
页码:3145 / 3148
页数:4
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