Lateral root formation and growth of Arabidopsis is redundantly regulated by cytokinin metabolism and signalling genes

被引:83
作者
Chang, Ling [1 ]
Ramireddy, Eswarayya [1 ]
Schmuelling, Thomas [1 ]
机构
[1] Free Univ Berlin, Inst Biol Appl Genet, DCPS, D-14195 Berlin, Germany
关键词
Arabidopsis thaliana; auxin; cytokinin; hormonal cross-talk; lateral root development; root system architecture; B RESPONSE REGULATORS; OVERLAPPING FUNCTIONS; AUXIN TRANSPORT; ABSCISIC-ACID; ENHANCED ROOT; FOUNDER CELLS; SHOOT GROWTH; INITIATION; REVEAL; CYCLE;
D O I
10.1093/jxb/ert291
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The plant root system is important for the uptake of water and nutrients and the anchoring of plants in the soil. Lateral roots (LRs) contribute considerably to root system architecture. Their post-embryonic formation is regulated by hormones and environmental cues. The hormone cytokinin influences LR formation and growth in Arabidopsis thaliana on different levels by disturbing cell division activity and pattern formation. This includes inhibition of the first formative cell division of pericycle founder cells and inhibition of the outgrowth of young LR primordia. Mutant analysis revealed that the cytokinin biosynthesis genes IPT3 and IPT5 and all three cytokinin receptor genes (AHK2, AHK3, and CRE1/AHK4) act redundantly during LR initiation. Mutation of AHK2 and AHK3 caused increased auxin sensitivity of LR formation, corroborating the functional relevance of auxin-cytokinin interaction during LR formation. In contrast, LR development of cytokinin receptor mutants in response to other hormones was mostly similar to that of the wild type, which is consistent with separate response pathways. A noticeable exception was an increased sensitivity of LR elongation to brassinolide in ahk2 ahk3 mutants indicating antagonistic action of cytokinin and brassinosteroid. It is proposed that the multilevel redundancy of the cytokinin system in modulating LR formation reflects its role in mediating environmental cues.
引用
收藏
页码:5021 / 5032
页数:12
相关论文
共 59 条
[1]   Environmental perception avenues: the interaction of cytokinin and environmental response pathways [J].
Argueso, Cristiana T. ;
Ferreira, Fernando J. ;
Kieber, Joseph J. .
PLANT CELL AND ENVIRONMENT, 2009, 32 (09) :1147-1160
[2]   Brassinosteroids interact with auxin to promote lateral root development in Arabidopsis [J].
Bao, F ;
Shen, JJ ;
Brady, SR ;
Muday, GK ;
Asami, T ;
Yang, ZB .
PLANT PHYSIOLOGY, 2004, 134 (04) :1624-1631
[3]   The peri-cell-cycle in Arabidopsis [J].
Beeckman, T ;
Burssens, S ;
Inzé, D .
JOURNAL OF EXPERIMENTAL BOTANY, 2001, 52 :403-411
[4]   Local, efflux-dependent auxin gradients as a common module for plant organ formation [J].
Benková, E ;
Michniewicz, M ;
Sauer, M ;
Teichmann, T ;
Seifertová, D ;
Jürgens, G ;
Friml, J .
CELL, 2003, 115 (05) :591-602
[5]   Lateral root organogenesis - from cell to organ [J].
Benkova, Eva ;
Bielach, Agnieszka .
CURRENT OPINION IN PLANT BIOLOGY, 2010, 13 (06) :677-683
[6]   Spatiotemporal Regulation of Lateral Root Organogenesis in Arabidopsis by Cytokinin [J].
Bielach, Agnieszka ;
Podlesakova, Katerina ;
Marhavy, Peter ;
Duclercq, Jerome ;
Cuesta, Candela ;
Mueller, Bruno ;
Grunewald, Wim ;
Tarkowski, Petr ;
Benkova, Eva .
PLANT CELL, 2012, 24 (10) :3967-3981
[7]   APICAL DOMINANCE IN ROOTS OF PISUM-SATIVUM-L [J].
BOTTGER, M .
PLANTA, 1974, 121 (03) :253-261
[8]   Transcript profiling of cytokinin action in Arabidopsis roots and shoots discovers largely similar but also organ-specific responses [J].
Brenner, Wolfram G. ;
Schmuelling, Thomas .
BMC PLANT BIOLOGY, 2012, 12
[9]   Auxin transport promotes Arabidopsis lateral root initiation [J].
Casimiro, I ;
Marchant, A ;
Bhalerao, RP ;
Beeckman, T ;
Dhooge, S ;
Swarup, R ;
Graham, N ;
Inzé, D ;
Sandberg, G ;
Casero, PJ ;
Bennett, M .
PLANT CELL, 2001, 13 (04) :843-852
[10]   Characterization of the response of the arabidopsis response regulator gene family to cytokinin [J].
D'Agostino, IB ;
Deruère, J ;
Kieber, JJ .
PLANT PHYSIOLOGY, 2000, 124 (04) :1706-1717