A molecular neuroanatomical study of the developing human neocortex from 8 to 17 postconceptional weeks revealing the early differentiation of the subplate and subventricular zone

被引:146
作者
Bayatti, Nadhim
Moss, Jennifer A.
Sun, Li [2 ]
Ambrose, Philip
Ward, Joseph F. H.
Lindsay, Susan [3 ]
Clowry, Gavin J. [1 ]
机构
[1] Royal Victoria Infirm, Sir James Spence Inst Child Hlth, Dept Child Hlth, Sch Clin Med Sci, Newcastle Upon Tyne NE1 4LP, Tyne & Wear, England
[2] Univ Newcastle, Inst Neurosci, Sch Biol & Psychol, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
[3] Univ Newcastle, Inst Human Genet, Newcastle Upon Tyne NE2 4HH, Tyne & Wear, England
基金
英国医学研究理事会; 英国惠康基金;
关键词
cell migration; cortical development; immunohistochemistry; synaptogenesis;
D O I
10.1093/cercor/bhm184
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
We have employed immunohistochemistry for multiple markers to investigate the structure and possible function of the different compartments of human cerebral wall from the formation of cortical plate at 8 postconceptional weeks (PCW) to the arrival of thalamocortical afferents at 17 PCW. New observations include the subplate emerging as a discrete differentiated layer by 10 PCW, characterized by synaptophysin and vesicular gamma-aminobutyric acid transporter expression also seen in the marginal zone, suggesting that these compartments may maintain a spontaneously active synaptic network even before the arrival of thalamocortical afferents. The subplate expanded from 13 to 17 PCW, becoming the largest compartment and differentiated further, with NPY neurons located in the outer subplate and KCC2 neurons in the inner subplate. Glutamate decarboxylase and calretinin-positive inhibitory neurons migrated tangentially and radially from 11.5 PCW, appearing in larger numbers toward the rostral pole. The proliferative zones, marked by Ki67 expression, developed a complicated structure by 12.5 PCW reflected in transcription factor expression patterns, including TBR2 confined to the inner subventricular and outer ventricular zones and TBR1 weakly expressed in the subventricular zone (SVZ). PAX6 was extensively expressed in the proliferative zones such that the human outer SVZ contained a large reservoir of PAX6-positive potential progenitor cells.
引用
收藏
页码:1536 / 1548
页数:13
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