Comparison of six methods for the extraction of lipids from serum in terms of effectiveness and protein preservation

被引：64

作者：

Ferraz, TPL

Fiúza, MC

dos Santos, MLA

de Carvalho, LP

Soares, NM

机构：

[1] Univ Fed Bahia, Fac Farm, Dept Anal Clin & Toxicol, BR-40170115 Salvador, BA, Brazil

[2] Fundacao Oswaldo Cruz, Ctr Pesquisa Goncalo Moniz, Salvador, BA, Brazil

来源：

JOURNAL OF BIOCHEMICAL AND BIOPHYSICAL METHODS | 2004年 / 58卷 / 03期

关键词：

lipid extraction; proteins; organic solvents; silica; detergent;

D O I：

10.1016/j.jbbm.2003.10.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present work compares six biochemical methods for extraction of lipids from human serum. Although some organic solvents were good lipid extractors, they precipitated most of the total proteins and albumin. On the other hand, methodologies using Triton X-114 and silica were efficient for extraction of lipids, while sparing the protein fraction. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：187 / 193

页数：7