Photodynamic assistance increases the efficiency of the process of microinjection in animal cells

被引：14

作者：

Saito, TK

Muguruma, H

Mabuchi, K

机构：

[1] Univ Tokyo, Ctr Collaborat Res, Meguro Ku, Tokyo 1538904, Japan

[2] Shibaura Inst Technol, Dept Elect Engn, Minato Ku, Tokyo 1088548, Japan

来源：

BIOTECHNOLOGY LETTERS | 2002年 / 24卷 / 04期

基金：

日本学术振兴会;

关键词：

alpha-terthienyl derivative; detachment assay; microinjection; photodynamic membrane damage; rat PC12 cell line;

D O I：

10.1023/A:1014072904009

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A microinjection process is described that involves the photodynamically-assisted puncture of an animal cell membrane by means of a brief and partial degeneration resulting from cell membrane damage. The ratio of dye-injected rat pheochromocytoma cells that became detached three and six days after photodynamically-assisted microinjection was 9%; however, it was 83% when a conventional mechanical microinjection process was used.

引用

页码：309 / 314

页数：6

共 17 条

[1]

ADAMO S, 1978, P AM ASSOC CANC RES, V19, P27

[2] TESTING THE INVIVO ROLE OF PROTEIN-KINASE-C AND C-FOS IN NEURITE OUTGROWTH BY MICROINJECTION OF ANTIBODIES INTO PC12-CELLS [J].