Simplified method to quantify furosemide in urine by high-performance liquid chromatography and ultraviolet detection

被引：24

作者：

Nava-Ocampo, AA

Velázquez-Armenta, EY

Reyes-Pérez, H

Ramirez-Lopez, E

Ponce-Monter, H

机构：

[1] Hosp Especialidades, Ctr Med Nacl Siglo XXI, Inst Mexicano Seguro Social, Unit Med Res Pharmacol, Mexico City 03020, DF, Mexico

[2] Hosp Infantil Mexico Federico Gomez, Dept Clin Pharmacol, Mexico City 06720, DF, Mexico

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 730卷 / 01期

关键词：

furosemide;

D O I：

10.1016/S0378-4347(99)00176-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Simplified reversed-phase high-performance liquid chromatographic method with ultraviolet detection at 280 nm without extraction procedure is described to quantify furosemide in rabbit and human urine. An internal standard was not used. The lower limit of quantitation was 0.750 mu g/ml using 50 mu l urine samples (100 mu l of total injection volume), and Linear response was tested from 0.750 to 250 mu g/ml in both humans and rabbits. Within and between-day accuracy and precision were always below 10% at all analyzed concentrations. Validation data showed that this method is linear, sensitive, selective, specific, accurate and reproducible. (C) 1999 Published by Elsevier Science B.V. All rights reserved.

引用

页码：49 / 54

页数：6

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