Borrelia burgdorferi NapA-Driven Th17 Cell Inflammation in Lyme Arthritis

被引：80

作者：

Codolo, Gaia ^{[2
]}

Amedei, Amedeo

Steere, Allen C. ^{[3
,4
]}

Papinutto, Elena ^{[2
,5
]}

Cappon, Andrea ^{[2
]}

Polenghi, Alessandra ^{[2
]}

Benagiano, Marisa

Paccani, Silvia Rossi ^{[6
]}

Sambri, Vittorio ^{[7
]}

Del Prete, Gianfranco

Baldari, Cosima Tatiana ^{[6
]}

Zanotti, Giuseppe ^{[2
]}

Montecucco, Cesare

D'Elios, Mario Milco ^{[1
,8
]}

de Bernard, Marina ^{[2
]}

机构：

[1] Univ Florence, Dept Internal Med, I-50134 Florence, Italy

[2] Univ Padua, Venetian Inst Mol Med, Padua, Italy

[3] Massachusetts Gen Hosp, Boston, MA 02114 USA

[4] Harvard Univ, Sch Med, Boston, MA USA

[5] CNR, Ist Chim Biomol, Padua Sect, Padua, Italy

[6] Univ Siena, I-53100 Siena, Italy

[7] Univ Bologna, Bologna, Italy

[8] Univ Careggi, Azienda Osped, Florence, Italy

来源：

ARTHRITIS AND RHEUMATISM | 2008年 / 58卷 / 11期

关键词：

D O I：

10.1002/art.23972

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

Objective. Human Lyme arthritis caused by Borrelia burgdorferi is characterized by an inflammatory infiltrate that consists mainly of neutrophils and T cells. This study was undertaken to evaluate the role of the innate and acquired immune responses elicited by the neutrophil-activating protein A (NapA) of B burgdorferi in patients with Lyme arthritis. Methods. Serum anti-NapA antibodies were measured in 27 patients with Lyme arthritis and 30 healthy control subjects. The cytokine profile of synovial fluid T cells specific for NapA was investigated in 5 patients with Lyme arthritis. The cytokine profile induced by NapA in neutrophils and monocytes was also investigated. Results. Serum anti-NapA antibodies were found in 48% of the patients with Lyme arthritis but were undetectable in the healthy controls. T cells from the synovial fluid of patients with Lyme arthritis produced interleukin-17 (IL-17) in response to NapA. Moreover, NapA was able to induce the expression of IL-23 in neutrophils and monocytes, as well as the expression of IL-6, IL-1 beta, and transforming growth factor beta (TGF beta) in monocytes, via Toll-like receptor 2. Conclusion. These findings indicate that NapA of B burgdorferi is able to drive the expression of IL-6, IL-1 beta, IL-23, and TGF beta by cells of the innate immune system and to elicit a synovial fluid Th17 cell response that might play a crucial role in the pathogenesis of Lyme arthritis.

引用

页码：3609 / 3617

页数：9

共 48 条

[1] The immunoglobulin (IgG) antibody response to OspA and OspB correlates with severe and prolonged Lyme arthritis and the IgG response to P35 correlates with mild and brief arthritis [J].