Measurement of cytokine release at the single cell level using the ELISPOT assay

被引：83

作者：

Cox, JH

Ferrari, G

Janetzki, S

机构：

[1] Henry M Jackson Fdn Adv Mil Med, US Mil HIV Res Program, Rockville, MD 20850 USA

[2] Duke Univ, Med Ctr, Dept Surg, Durham, NC 27710 USA

[3] ZellNet Consulting Inc, Ft Lee, NJ 07024 USA

来源：

METHODS | 2006年 / 38卷 / 04期

关键词：

ELISPOT; interferon-gamma; interleukin-2; cytokine secretion; validation;

D O I：

10.1016/j.ymeth.2005.11.006

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The enzyme-linked immunospot (ELISPOT) assay took its design concept from traditional ELISA techniques and evolved over the years from a method for detecting antibodies secreted from B cells to a method for detecting cytokines or other soluble mediators secreted from a variety of different cell types. The ELISPOT assay allows the quantitative measurement of frequency of cytokine secreting cells at the single cell level directly ex vivo without elaborate in vitro expansion or manipulation of cell populations. The function of cells can be inferred from the pattern of cytokines secreted by cells in response to diverse antigenic Stimuli and thus the ELISPOT assay has become a powerful method for monitoring immune responses in health and disease. The ELISPOT assay like the ELISA assay is relatively easy to perform and it has the promise of robustness, reliability, and reproducibility of performance for use as a diagnostic tool. The history, applications, validation process, and future challenges of the ELISPOT assay are discussed in this chapter. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：274 / 282

页数：9

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