Cloning and expression of human beta-defensin-2 gene in Escherichia coli

被引：31

作者：

Fang, XM

Peng, L

Xu, ZN

Wu, JM

Cen, PL

机构：

[1] Zhejiang Univ, Inst Bioengn, Dept Chem Engn & Bioengn, Hangzhou 310027, Peoples R China

[2] Zhejiang Univ, Sch Med Sci, Sir Run Run Shaw Hosp, Hangzhou 310027, Peoples R China

来源：

PROTEIN AND PEPTIDE LETTERS | 2002年 / 9卷 / 01期

关键词：

antimicrobial peptide; human beta-defensin-2; fusion protein expression;

D O I：

10.2174/0929866023409011

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.

引用

页码：31 / 37

页数：7

共 7 条

[1] Human β-defensin 2 is a salt-sensitive peptide antibiotic expressed in human lung [J].