Gene targeting restricted to mouse striated muscle lineage

被引：165

作者：

Miniou, Pierre ^{[1
]}

Tiziano, Danilo ^{[1
]}

Frugier, Tony ^{[1
]}

Roblot, Natacha ^{[1
]}

Le Meur, Marianne ^{[1
]}

Melki, Judith ^{[1
]}

机构：

[1] CU Strasbourg, CNRS, INSERM, ULP,IGBMC, F-67404 Illkirch Graffenstaden, France

来源：

NUCLEIC ACIDS RESEARCH | 1999年 / 27卷 / 19期

关键词：

D O I：

10.1093/nar/27.19.e27

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Spatially and temporally regulated somatic mutations can be achieved by using the Cre/LoxP recombination system of bacteriophage P1. In order to develop gene knockouts restricted to striated muscle, we generated a transgenic mouse line expressing Cre recombinase under the control of the human alpha-skeletal actin promoter. Specific excision of a loxP-flanked gene was demonstrated in striated muscle, heart and skeletal muscle, in a pattern very similar to the expression of the endogenous alpha-skeletal actin gene. Therefore, the reported transgenic line can be used to target inactivation or activation of a given gene to the skeletal muscle lineage.

引用

页数：4

共 11 条

[1] Cre-mediated somatic site-specific recombination in mice [J].

Akagi, K ;

Sandig, V ;

Vooijs, M ;

VanderValk, M ;

Giovannini, M ;

Strauss, M ;

Berns, A .

NUCLEIC ACIDS RESEARCH, 1997, 25 (09) :1766-1773

[2] Developmental and tissue-specific regulation of the murine cardiac actin gene in vivo depends on distinct skeletal and cardiac muscle-specific enhancer elements in addition to the proximal promoter [J].

Biben, C ;

Hadchouel, J ;

Tajbakhsh, S ;

Buckingham, M .

DEVELOPMENTAL BIOLOGY, 1996, 173 (01) :200-212

[3]

BRENNAN KJ, 1993, J BIOL CHEM, V268, P719

[4]

HOGAN B, 1994, MANIPULATING MOUSE E, P226

[5] MULTIPLE 5'-FLANKING REGIONS OF THE HUMAN ALPHA-SKELETAL ACTIN GENE SYNERGISTICALLY MODULATE MUSCLE-SPECIFIC EXPRESSION [J].

MUSCAT, GEO ;

KEDES, L .

MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (11) :4089-4099

[6]

OPPENHEIM RW, 1991, ANNU REV NEUROSCI, V14, P453, DOI 10.1146/annurev.ne.14.030191.002321

[7]

SASSOON DA, 1988, DEVELOPMENT, V104, P155

[8] SITE-SPECIFIC DNA RECOMBINATION IN MAMMALIAN-CELLS BY THE CRE RECOMBINASE OF BACTERIOPHAGE-P1 [J].

SAUER, B ;

HENDERSON, N .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (14) :5166-5170

[9] BACTERIOPHAGE-P1 SITE-SPECIFIC RECOMBINATION .1. RECOMBINATION BETWEEN LOXP SITES [J].

STERNBERG, N ;

HAMILTON, D .

JOURNAL OF MOLECULAR BIOLOGY, 1981, 150 (04) :467-486

[10] SITE-DIRECTED MUTAGENESIS BY GENE TARGETING IN MOUSE EMBRYO-DERIVED STEM-CELLS [J].

THOMAS, KR ;

CAPECCHI, MR .

CELL, 1987, 51 (03) :503-512

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