Nutrient composition, polyphenolic contents, and in situ protein degradation kinetics of leaves from three mulberry species

Mulberry leaves of 14 cultivars of Morus atroputpurea Roxb (MAR), 7 cultivars of Morus alba Linn (MAL) and 8 cultivars of Morus multicaulis Pert (MMP) were assessed for their differences in chemical composition and in situ ruminal degradation. The contents of acid detergent fibre (P=0.073) and ether extract (P < 0.0001) ranked as: MMP > MAL > MAR. Crude protein (CP), neutral detergent fibre and polyphenolic contents of the leaves did not differ between three species. Nylon bags containing the leaves were incubated for 2, 6, 12, 24, 36, 48 and 72 h in three rumen cannulated dairy cows. Soluble CP fraction and fractional degradation rate did not differ between three species, but the potential degradable CP fraction in the leaves was greater for MAR than the others (P=0.005). Effective CP degradability was consistently ranked: MAR > MAL > MMP (P=0.051). Non-tannin phenols and total tannins were negatively correlated with the soluble CP fraction (r = -0.48, P < 0.01) and the potential degradable CP fraction (r = -0.36, P < 0.05), respectively. In summary, the mulberry leaves with high protein, low fibre and tannin contents could be exceptional forage for use in ruminant animals. (C) 2012 Elsevier B.V. All rights reserved.