A new fluorescent biosensor for inositol trisphosphate

被引：52

作者：

Morii, T ^{[1
]}

Sugimoto, K

Makino, K

Otsuka, M

Imoto, K

Mori, Y

机构：

[1] Kyoto Univ, PRESTO, Japan Sci & Technol Corp, Inst Adv Energy, Kyoto 6110011, Japan

[2] Kumamoto Univ, Grad Sch Pharmaceut Sci, Fac Bioorgan Med Chem, Kumamoto 8620973, Japan

[3] Natl Inst Physiol Sci, Dept Informat Physiol, Okazaki, Aichi 4448585, Japan

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2002年 / 124卷 / 07期

关键词：

D O I：

10.1021/ja016824d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An intracellular second messenger d-myo-inositol-1,4,5-trisphosphate (IP3) is a key biological signaling molecule that controls the cellular Ca2+ concentration. We report the preparation and evaluation of a functionalized protein-based sensor for IP3 by exploring the selective IP3 binding properties of pleckstrin homology (PH) domain. Signal transduction is imparted to the protein by mutation of proximal residues to cysteine and then alkylation of the active site by various fluorophore derivatives. This creates functionalized proteins that show micromolar affinity for IP3, reasonably strong fluorescence emission, and wavelength changes in the fluorophore and selectivity higher than the original PH domain among different inositol phosphate derivatives. Copyright © 2002 American Chemical Society.

引用

页码：1138 / 1139

页数：2

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